Biological and phylogenetic characteristics of West African lineages of West Nile

Autor(es)

Fall, Gamou ^[1] ; Di Paola, Nicholas ^[2] ; Faye, Martin ^[1] ; Dia, Moussa ^[1] ; de Melo Freire, Caio Cesar ^[3] ; Loucoubar, Cheikh ^[4] ; de Andrade Zanotto, Paolo Marinho ^[2] ; Faye, Ousmane ^[1] ; Sall, Amadou Alpha ^[1]

Afiliação do(s) autor(es)

^[1] Inst Pasteur, Unite Arbovirus & Virus Fievres Hemorrag, Pole Virol, Dakar - Senegal

^[2] Univ Sao Paulo, Biomed Sci Inst, Dept Microbiol, Lab Mol Evolut & Bioinformat, Sao Paulo - Brazil

^[3] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos, SP - Brazil

^[4] Inst Pasteur, Grp Ans Biostat Bioinformat & Modelisat 4, Dakar - Senegal

• Tipo de documento: Artigo Científico
• Fonte: PLoS Neglected Tropical Diseases; v. 11, n. 11 NOV 2017.
• Citações Web of Science: 16

Resumo

The West Nile virus (WNV), isolated in 1937, is an arbovirus (arthropod-borne virus) that infects thousands of people each year. Despite its burden on global health, little is known about the virus' biological and evolutionary dynamics. As several lineages are endemic in West Africa, we obtained the complete polyprotein sequence from three isolates from the early 1990s, each representing a different lineage. We then investigated differences in growth behavior and pathogenicity for four distinct West African lineages in arthropod (Ap61) and primate (Vero) cell lines, and in mice. We found that genetic differences, as well as viral-host interactions, could play a role in the biological properties in different WNV isolates in vitro, such as: (i) genome replication, (ii) protein translation, (iii) particle release, and (iv) virulence. Our findings demonstrate the endemic diversity of West African WNV strains and support future investigations into (i) the nature of WNV emergence, (ii) neurological tropism, and (iii) host adaptation. (AU)

• Processo FAPESP: 14/17766-9 - Abordagem sistêmica no estudo da permissividade do Anticarsia gemmatalis múltiplo nucleopoliedrovírus (AgMNPV)
• Beneficiário: Paolo Marinho de Andrade Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/22136-1 - Aplicação de metagenômica para descoberta de novos vírus na Mata Atlântica paulista
• Beneficiário: Nicholas Di Paola
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto