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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biological and phylogenetic characteristics of West African lineages of West Nile

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Author(s):
Fall, Gamou [1] ; Di Paola, Nicholas [2] ; Faye, Martin [1] ; Dia, Moussa [1] ; de Melo Freire, Caio Cesar [3] ; Loucoubar, Cheikh [4] ; de Andrade Zanotto, Paolo Marinho [2] ; Faye, Ousmane [1] ; Sall, Amadou Alpha [1]
Total Authors: 9
Affiliation:
[1] Inst Pasteur, Unite Arbovirus & Virus Fievres Hemorrag, Pole Virol, Dakar - Senegal
[2] Univ Sao Paulo, Biomed Sci Inst, Dept Microbiol, Lab Mol Evolut & Bioinformat, Sao Paulo - Brazil
[3] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos, SP - Brazil
[4] Inst Pasteur, Grp Ans Biostat Bioinformat & Modelisat 4, Dakar - Senegal
Total Affiliations: 4
Document type: Journal article
Source: PLoS Neglected Tropical Diseases; v. 11, n. 11 NOV 2017.
Web of Science Citations: 16
Abstract

The West Nile virus (WNV), isolated in 1937, is an arbovirus (arthropod-borne virus) that infects thousands of people each year. Despite its burden on global health, little is known about the virus' biological and evolutionary dynamics. As several lineages are endemic in West Africa, we obtained the complete polyprotein sequence from three isolates from the early 1990s, each representing a different lineage. We then investigated differences in growth behavior and pathogenicity for four distinct West African lineages in arthropod (Ap61) and primate (Vero) cell lines, and in mice. We found that genetic differences, as well as viral-host interactions, could play a role in the biological properties in different WNV isolates in vitro, such as: (i) genome replication, (ii) protein translation, (iii) particle release, and (iv) virulence. Our findings demonstrate the endemic diversity of West African WNV strains and support future investigations into (i) the nature of WNV emergence, (ii) neurological tropism, and (iii) host adaptation. (AU)

FAPESP's process: 14/17766-9 - A systemic approach to study permissivity on the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV)
Grantee:Paolo Marinho de Andrade Zanotto
Support Opportunities: Regular Research Grants
FAPESP's process: 13/22136-1 - Discovering novel viruses in communities encroaching tropical hotspots via a metagenomics approach
Grantee:Nicholas Di Paola
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)