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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Photobiomodulation of mesenchymal stem cells encapsulated in an injectable rhBMP4-loaded hydrogel directs hard tissue bioengineering

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Autor(es):
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Diniz, Ivana M. A. [1] ; Carreira, Ana C. O. [2, 3] ; Sipert, Carla R. [4] ; Uehara, Cindi M. [4] ; Moreira, Maria S. N. [5] ; Freire, Laila [4] ; Pelissari, Cibele [6] ; Kossugue, Patricia M. [2, 3] ; de Araujo, Daniele R. [7] ; Sogayar, Mari C. [2, 3] ; Marques, Marcia M. [4]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Fed Minas Gerais, Dept Restorat Dent, Sch Dent, Ave Antonio Carlos 6627, BR-31279910 Belo Horizonte, MG - Brazil
[2] Univ Sao Paulo, Cell & Mol Therapy Ctr NUCEL NETCEM, Sch Med, Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Biochem, Chem Inst, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Restorat Dent, Sch Dent, Sao Paulo - Brazil
[5] Univ Ibirapuera, Dept Biodent, Sch Dent, Sao Paulo - Brazil
[6] Univ Sao Paulo, Dept Stomatol, Sch Dent, Sao Paulo - Brazil
[7] ABC Fed Univ, Nat & Human Sci Ctr, Santo Andre - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: Journal of Cellular Physiology; v. 233, n. 6, p. 4907-4918, JUN 2018.
Citações Web of Science: 9
Resumo

Photobiomodulation (PBM) therapy displays relevant properties for tissue healing and regeneration, which may be of interest for the tissue engineering field. Here, we show that PBM is able to improve cell survival and to interact with recombinant human Bone Morphogenetic Protein 4 (rhBMP4) to direct and accelerate odonto/osteogenic differentiation of dental derived mesenchymal stem cells (MSCs). MSCs were encapsulated in an injectable and thermo-responsive cell carrier (Pluronic((R)) F-127) loaded with rhBMP4 and then photoactivated. PBM improved MSCs self-renewal and survival upon encapsulation in the Pluronic((R)) F-127. In the presence of rhBMP4, cell odonto/osteogenic differentiation was premature and markedly improved in the photoactivated MSCs. An in vivo calvarial critical sized defect model demonstrated significant increase in bone formation after PBM treatment. Finally, a balance in the reactive oxygen species levels may be related to the favorable results of PBM and rhBMP4 association. PBM may act in synergism with rhBMP4 and is a promise candidate to direct and accelerate hard tissue bioengineering. (AU)

Processo FAPESP: 12/16552-0 - Efeitos da fototerapia com laser em baixa intensidade associada a scaffold com incorporação de BMP4 na diferenciação e migração de células-tronco da polpa dentária humana. Estudo in vitro.
Beneficiário:Ivana Marcia Alves Diniz
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/17268-6 - Efeito da fototerapia a laser no transplante de hydrogel com incorporação de BMP4 e céluas-tronco para a regeneração do complexo dentino-pulpar in vivo
Beneficiário:Ivana Marcia Alves Diniz
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado