Diniz, Ivana M. A.
Carreira, Ana C. O.
Sipert, Carla R.
Uehara, Cindi M.
Moreira, Maria S. N.
Kossugue, Patricia M.
de Araujo, Daniele R.
Sogayar, Mari C.
Marques, Marcia M.
Total Authors: 11
 Univ Fed Minas Gerais, Dept Restorat Dent, Sch Dent, Ave Antonio Carlos 6627, BR-31279910 Belo Horizonte, MG - Brazil
 Univ Sao Paulo, Cell & Mol Therapy Ctr NUCEL NETCEM, Sch Med, Sao Paulo - Brazil
 Univ Sao Paulo, Dept Biochem, Chem Inst, Sao Paulo - Brazil
 Univ Sao Paulo, Dept Restorat Dent, Sch Dent, Sao Paulo - Brazil
 Univ Ibirapuera, Dept Biodent, Sch Dent, Sao Paulo - Brazil
 Univ Sao Paulo, Dept Stomatol, Sch Dent, Sao Paulo - Brazil
 ABC Fed Univ, Nat & Human Sci Ctr, Santo Andre - Brazil
Total Affiliations: 7
Journal of Cellular Physiology;
Web of Science Citations:
Photobiomodulation (PBM) therapy displays relevant properties for tissue healing and regeneration, which may be of interest for the tissue engineering field. Here, we show that PBM is able to improve cell survival and to interact with recombinant human Bone Morphogenetic Protein 4 (rhBMP4) to direct and accelerate odonto/osteogenic differentiation of dental derived mesenchymal stem cells (MSCs). MSCs were encapsulated in an injectable and thermo-responsive cell carrier (Pluronic((R)) F-127) loaded with rhBMP4 and then photoactivated. PBM improved MSCs self-renewal and survival upon encapsulation in the Pluronic((R)) F-127. In the presence of rhBMP4, cell odonto/osteogenic differentiation was premature and markedly improved in the photoactivated MSCs. An in vivo calvarial critical sized defect model demonstrated significant increase in bone formation after PBM treatment. Finally, a balance in the reactive oxygen species levels may be related to the favorable results of PBM and rhBMP4 association. PBM may act in synergism with rhBMP4 and is a promise candidate to direct and accelerate hard tissue bioengineering. (AU)