Interaction of pDNA with reverse phase chitosome

Autor(es)

Mertins, Omar ^[1] ; Lobo, Sonja Ellen ^[2] ; Mathews, Patrick D. ^[1] ; Han, Sang Won ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Paulista Sch Med, Dept Biophys, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Paulista Sch Med, Interdisciplinary Ctr Gene Therapy CINTERGEN, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS; v. 543, p. 76-82, APR 20 2018.
• Citações Web of Science: 0
• Assunto(s): Nanopartículas Quitosana Lipossomos

Resumo

The use of liposomes and polyelectrolytes is on scientific and technologic scrutiny for development of gene nano carriers. We produced a reverse phase lipid: chitosan ``chitosome{''} to which plasmid DNA (pDNA) effectively interacts and compacts. Dynamic light scattering and zeta potential evidence the complexation between pDNA and lipid: chitosan particles with size increase and surface charge decrease with concentration of plasmid. Zeta potential shows a variation of -4 mV/mu g plasmid. Cell culture media have weak or even absent effect on size and surface charge of the composite complexes denoting physical stability in application media. SAXS results show increased structural organization of the lipopolyplex, with a membrane repeat distance of 6.50 nm, and gel electrophoresis confirms effective interaction between chitosomes and pDNA. The thermodynamic energy involved in complexation provides 0.29 chitosan protonated monomers interacting to phosphate group of pDNA at saturation as determined by isothermal titration calorimetry, which is in accordance with the linear charge density between chitosan and DNA. The strong exothermic binding determines Gibbs energy around -13.45 kcal/mol of protonated chitosan denoting that the complex formation leads to a state of lower Gibbs energy, thus energetically favored with decrease in entropy, corroborating compaction of plasmid chains over surface of chitosomes. A model of the lipopolyplex is unveiled thanks to the complete physicochemical description. The results demonstrate perspectives for development of a promising gene deliver. (AU)

• Processo FAPESP: 16/13368-4 - Sistemas nanoestruturados: de modelos biomiméticos de membranas a carreadores de bioativos
• Beneficiário: Karin Do Amaral Riske
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/20206-8 - Modulação de monócitos, macrófagos e pericitos pelos genes dos fatores estimuladores de colônia para tratamento de isquemia de membros em modelo murino
• Beneficiário: Sang Won Han
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/23948-5 - Aperfeiçoamento das propriedades do polissacarídeo quitosana para aplicação em lipossomas e vesículas gigantes
• Beneficiário: Omar Mertins
• Modalidade de apoio: Auxílio à Pesquisa - Regular