Potential of Mangrove-Associated Endophytic Fungi for Production of Carbohydrolases with High Saccharification Efficiency

Autor(es)

Maroldi, M. M. C. ^{[1, 2]} ; Vasconcellos, V. M. ^{[1, 3]} ; Lacava, P. T. ^[2] ; Farinas, C. S. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Embrapa Instrumentat, Rua 15 Novembro 1452, BR-13561206 Sao Paulo - Brazil

^[2] Univ Fed Sao Carlos, Ctr Biol Sci & Hlth, POB 676, BR-13565905 Sao Paulo - Brazil

^[3] Univ Fed Sao Carlos, Grad Program Chem Engn, POB 676, BR-13565905 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 184, n. 3, p. 806-820, MAR 2018.
• Citações Web of Science: 3

Resumo

The endophytic fungi represent a potential source of microorganisms for enzyme production. However, there have been only few studies exploiting their potential for the production of enzymes of industrial interest, such as the (hemi)cellulolytic enzymatic cocktail required in the hydrolysis of lignocellulosic biomass. Here, a collection of endophytic fungi isolated from mangrove tropical forests was evaluated for the production of carbohydrolases and performance on the hydrolysis of cellulose. For that, 41 endophytic strains were initially screened using a plate assay containing crystalline cellulose as the sole carbon source and the selected strains were cultivated under solid-state fermentation for endoglucanase, beta-glucosidase, and xylanase enzyme quantification. The hydrolysis of a cellulosic material with the enzymes from endophytic strains of the Aspergillus genus resulted in glucose and conversion values more than twofold higher than the reference strains (Aspergillus niger F12 and Trichoderma reesei Rut-C30). Particularly, the enzymes from strains A. niger 56 (3) and A. awamori 82 (4) showed a distinguished saccharification performance, reaching cellulose conversion values of about 35% after 24 h. Linking hydrolysis performance to the screening steps played an important role towards finding potential fungal strains for producing enzymatic cocktails with high saccharification efficiency. These results indicate the potential of mangrove-associated endophytic fungi for production of carbohydrolases with efficient performance in the hydrolysis of biomass, thus contributing to the implementation of future biorefineries. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 06/57060-1 - Análise da comunidade bacteriana associada a manguezais do estado de São Paulo com interesse biotecnológico e agrícola
• Beneficiário: Paulo Teixeira Lacava
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 04/13910-6 - Biodiversidade e atividades funcionais de microrganismos de manguezais do estado de São Paulo
• Beneficiário: Itamar Soares de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 14/19000-3 - Simplificação do processo de conversão de biomassa a etanol por meio da integração da produção de enzimas in-house
• Beneficiário: Cristiane Sanchez Farinas
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular