Apoptotic intrinsic pathway proteins predict survival in canine cutaneous mast cell tumours

Autor(es):Mostrar menos -

Barra, C. N. ^{[1, 2]} ; Macedo, B. M. ^[2] ; Cadrobbi, K. G. ^[2] ; Pulz, L. H. ^{[1, 2]} ; Huete, G. C. ^[2] ; Kleeb, S. R. ^[3] ; Xavier, J. G. ^[4] ; Catao-Dias, J. L. ^[1] ; Nishiya, A. T. ^[5] ; Fukumasu, H. ^[2] ; Strefezzi, R. F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pathol, Fac Med Vet & Zootecnia, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Lab Oncol Comparada & Translac, Fac Zootecnia & Engn Alimentos, Pirassununga - Brazil

^[3] Univ Metodista Sao Paulo, Sao Bernardo Do Campo - Brazil

^[4] Univ Paulista, Sao Paulo - Brazil

^[5] Univ Anhembi Morumbi, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY AND COMPARATIVE ONCOLOGY; v. 16, n. 1, p. E38-E44, MAR 2018.
• Citações Web of Science: 2

Resumo

Mast cell tumours (MCTs) are the most frequent canine round cell neoplasms and show variable biological behaviours with high metastatic and recurrence rates. The disease is treated surgically and wide margins are recommended. Adjuvant chemotherapy and radiotherapy used in this disease cause DNA damage in neoplastic cells, which is aimed to induce apoptotic cell death. Resisting cell death is a hallmark of cancer, which contributes to the development and progression of tumours. The aim of this study was to investigate the expression of the proteins involved in the apoptotic intrinsic pathway and to evaluate their potential use as prognostic markers for canine cutaneous MCTs. Immunohistochemistry for BAX, BCL2, APAF1, Caspase-9, and Caspase-3 was performed in 50 canine cases of MCTs. High BAX expression was associated with higher mortality rate and shorter survival. BCL2 and APAF1 expressions offered additional prognostic information to the histopathological grading systems. The present results indicate that variations in the expression of apoptotic proteins are related to malignancy of cutaneous MCTs in dogs. (AU)

• Processo FAPESP: 13/13252-8 - Caracterização de genes relacionados à apoptose em mastocitomas cutâneos caninos e seu valor como indicador prognóstico
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/03862-1 - Caracterização de fibroblastos associados ao câncer e sua influência sobre neoplasias caninas
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular