Physical exercise alters the activation of downstream proteins related to BDNF-TrkB signaling in male Wistar rats with epilepsy

Autor(es)

de Almeida, Alexandre Aparecido ^{[1, 2]} ; da Silva, Sergio Gomes ^{[3, 4]} ; Lopim, Glauber Menezes ^[1] ; Campos, Diego Vannucci ^[1] ; Fernandes, Jansen ^[1] ; Cabral, Francisco Romero ^{[3, 5]} ; Arida, Ricardo Mario ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Fisiol, Rua Botucatu 862, Ed Ciencias Biomed, 5 Andar, BR-04023900 Sao Paulo, SP - Brazil

^[2] Inst Fed Goiano, Campus Ceres, Ceres - Brazil

^[3] Hosp Israelita Albert Einstein, Sao Paulo - Brazil

^[4] Univ Mogi das Cruzes, Mogi Das Cruzes - Brazil

^[5] Santa Casa Sao Paulo, Fac Ciencias Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Neuroscience Research; v. 96, n. 5, p. 911-920, MAY 2018.
• Citações Web of Science: 4

Resumo

There are a considerable number of studies concerning the behavioral effects of physical exercise on the epileptic brain; however, the intracellular signaling mechanisms involved remain unclear. We investigated the effects of aerobic exercise on hippocampal levels of brain-derived neurotrophic factor (BDNF), expression of its receptor tropomyosin receptor kinase B (TrkB), and activation of intracellular proteins related to BDNF-TrkB signaling in male Wistar rats with pilocarpine-induced epilepsy. Thirty days after the first spontaneous seizure, rats from the exercise group undertook a 30-day physical exercise program on the treadmill. Thereafter, BDNF levels, expression of TrkB, and activation of intracellular proteins were quantified by enzyme-linked immunosorbent assay, Western blotting, and multiplex assay, respectively. Statistical analyses were conducted using nonparametric tests. Rats with epilepsy presented decreased BDNF levels compared with control rats. BDNF levels increased significantly in the exercise group compared with the epileptic and control groups. Expression of full-length and truncated TrkB was increased in rats with epilepsy, and physical exercise restored its expression to control levels. RAC-alpha serine/threonine-protein kinase, mammalian target of rapamycin, and extracellular signal-regulated kinase activation were reduced in rats with epilepsy, and exercise increased activation compared with control and epilepsy groups. Increased cAMP response element binding protein activation was observed in the exercise group compared with the epilepsy group. Our findings indicate that the beneficial effects of exercise in the epileptic brain can be in part related to alterations in the activation of proteins related to the BDNF-TrkB signaling pathway. (AU)

• Processo FAPESP: 13/12692-4 - Influência do exercício aeróbico sobre a expressão e a distribuição de microRNAs na formação hipocampal de ratos adultos
• Beneficiário: Ricardo Mario Arida
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/00035-1 - Reserva neural induzida pela prática de atividade física na adolescência: um estudo do fator neurotrófico derivado do encéfalo (BDNF), de seus receptores (TrkB e P75ntr) e vias de sinalização intracelular
• Beneficiário: Sérgio Gomes da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/13725-3 - Influência do Exercício Aeróbico Sobre a Expressão e a Distribuição de microRNAs na Formação Hipocampal de Ratos Adultos
• Beneficiário: Jansen Fernandes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50680-2 - Investigação multimodal da epileptogênese com ênfase na incorporação de novos modelos e novas ferramentas
• Beneficiário: Iscia Teresinha Lopes Cendes
• Modalidade de apoio: Auxílio à Pesquisa - Temático