Biopolymer-based membranes associated with osteogenic growth peptide for guided bone regeneration

Autor(es):Mostrar menos -

Saska, Sybele ^{[1, 2]} ; Pigossi, Suzane C. ^{[2, 3]} ; Oliveira, Guilherme J. P. L. ^[2] ; Teixeira, Lucas N. ^[4] ; Capela, Marisa V. ^[1] ; Goncalves, Andreia ^[2] ; de Oliveira, Paulo T. ^[4] ; Messaddeq, Younes ^[1] ; Ribeiro, Sidney J. L. ^[1] ; Minarelli Gaspar, Ana Maria ^[2] ; Marchetto, Reinaldo ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Sch Dent, Araraquara, SP - Brazil

^[3] Alfenas Fed Univ UNIFAL MG, Sch Dent, Alfenas, MG - Brazil

^[4] Univ Sao Paulo, Fac Dent Ribeirao Preto, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biomedical Materials; v. 13, n. 3 MAY 2018.
• Citações Web of Science: 1

Resumo

Barrier membranes for guided bone regeneration (GBR) mainly promote mechanical maintenance of bone defect space and induce osteopromotion. Additionally, biopolymer-based membranes may provide greater bioactivity and biocompatibility due to their similarity to extracellular matrix (ECM). In this study, biopolymers-based membranes from bacterial cellulose (BC) and collagen (COL) associated with osteogenic growth peptide (OGP(10-14)) were evaluated to determine in vitro osteoinductive potential in early osteogenesis; moreover, histological study was performed to evaluate the BC-COL OGP(10-14) membranes on bone healing after GBR in noncritical defects in rat femur. The results showed that the BC-COL and BC-COL OGP(10-14) membranes promoted cell proliferation and alkaline phosphatase activity in osteoblastic cell cultures. However, ECM mineralization was similar between cultures grown on BCOGP(10-14) and BC-COLOGP(10-14) membranes. In vivo results showed that all the membranes tested, including the peptide-free BC membrane, promoted better bone regeneration than control group. Furthermore, the BC-COLOGP(10-14) membranes induced higher radiographic density in the repaired bone than the other groups at 1, 4 and 16 weeks. Histomorphometric analyses revealed that the BC-COLOGP(10-14) induced higher percentage of bone tissue in the repaired area at 2 and 4 weeks than others membranes. In general, these biopolymer-based membranes might be potential candidates for bone regeneration applications. (AU)

• Processo FAPESP: 09/09960-1 - Nanocompositos à base de celulose bacteriana para aplicação na regeneração do tecido ósseo.
• Beneficiário: Reinaldo Marchetto
• Modalidade de apoio: Auxílio à Pesquisa - Regular