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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biopolymer-based membranes associated with osteogenic growth peptide for guided bone regeneration

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Author(s):
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Saska, Sybele [1, 2] ; Pigossi, Suzane C. [2, 3] ; Oliveira, Guilherme J. P. L. [2] ; Teixeira, Lucas N. [4] ; Capela, Marisa V. [1] ; Goncalves, Andreia [2] ; de Oliveira, Paulo T. [4] ; Messaddeq, Younes [1] ; Ribeiro, Sidney J. L. [1] ; Minarelli Gaspar, Ana Maria [2] ; Marchetto, Reinaldo [1]
Total Authors: 11
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil
[2] Sao Paulo State Univ Unesp, Sch Dent, Araraquara, SP - Brazil
[3] Alfenas Fed Univ UNIFAL MG, Sch Dent, Alfenas, MG - Brazil
[4] Univ Sao Paulo, Fac Dent Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Biomedical Materials; v. 13, n. 3 MAY 2018.
Web of Science Citations: 1
Abstract

Barrier membranes for guided bone regeneration (GBR) mainly promote mechanical maintenance of bone defect space and induce osteopromotion. Additionally, biopolymer-based membranes may provide greater bioactivity and biocompatibility due to their similarity to extracellular matrix (ECM). In this study, biopolymers-based membranes from bacterial cellulose (BC) and collagen (COL) associated with osteogenic growth peptide (OGP(10-14)) were evaluated to determine in vitro osteoinductive potential in early osteogenesis; moreover, histological study was performed to evaluate the BC-COL OGP(10-14) membranes on bone healing after GBR in noncritical defects in rat femur. The results showed that the BC-COL and BC-COL OGP(10-14) membranes promoted cell proliferation and alkaline phosphatase activity in osteoblastic cell cultures. However, ECM mineralization was similar between cultures grown on BCOGP(10-14) and BC-COLOGP(10-14) membranes. In vivo results showed that all the membranes tested, including the peptide-free BC membrane, promoted better bone regeneration than control group. Furthermore, the BC-COLOGP(10-14) membranes induced higher radiographic density in the repaired bone than the other groups at 1, 4 and 16 weeks. Histomorphometric analyses revealed that the BC-COLOGP(10-14) induced higher percentage of bone tissue in the repaired area at 2 and 4 weeks than others membranes. In general, these biopolymer-based membranes might be potential candidates for bone regeneration applications. (AU)

FAPESP's process: 09/09960-1 - Nanocomposites based on bacterial cellulose for application in the bone tissue regeneration
Grantee:Reinaldo Marchetto
Support Opportunities: Regular Research Grants