Phytophthora parasitica Effector PpRxLR2 Suppresses Nicotiana benthamiana Immunity

Autor(es)

Dalio, R. J. D. ^[1] ; Maximo, H. J. ^[1] ; Oliveira, T. S. ^[1] ; Dias, R. O. ^[2] ; Breton, M. C. ^[1] ; Felizatti, H. ^[3] ; Machado, M. ^[1]

Afiliação do(s) autor(es)

^[1] Ctr Citricultura Sylvio Moreira, Inst Agron, Biotechnol Lab, Cordeiropolis, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim, Sao Paulo, SP - Brazil

^[3] Univ Estadual Campinas Unicamp, Inst Matemat Fis & Comp Cient, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR PLANT-MICROBE INTERACTIONS; v. 31, n. 4, p. 481-493, APR 2018.
• Citações Web of Science: 5

Resumo

Phytophthora species secrete several classes of effector proteins during interaction with their hosts. These proteins can have multiple functions including modulation of host physiology and immunity. The RxLR effectors have the ability to enter plant cells using the plant machinery. Some of these effectors have been characterized as immunity suppressors; however, very little is known about their functions in the interaction between Phytophthora parasitica and its hosts. Using a bioinformatics pipeline, we have identified 172 candidate RxLR effectors (CREs) in the isolate IAC 01\_95 of P. parasitica. Of these 172 CREs, 93 were found to be also present in eight other genomes of P. parasitica, isolated from different hosts and continents. After transcriptomics and gene expression analysis, we have found five CREs to be up-regulated in in-vitro and in-planta samples. Subsequently, we selected three CREs for functional characterization in the model plant Nicotiana benthamiana. We show that PpRxLR2 is able to completely suppress INF-1-induced cell death, whereas PpRxLR3 and PpRxLR5 moderately suppressed N. benthamiana immunity in a less-extensive manner. Moreover, we confirmed the effector-triggered susceptibility activity of these proteins after transient transformation and infection of N. benthamiana plants. All three CREs enhanced virulence of P. parasitica during the interaction with N. benthamiana. These effectors, in particular PpRxLR2, can be targeted for the development of biotechnology-based control strategies of P. parasitica diseases. (AU)

• Processo FAPESP: 15/14498-6 - Efetorômica: efetores como novas ferramentas para o controle de Phytophthora parasitica, agente da gomose e podridão de raízes dos citros.
• Beneficiário: Ronaldo José Durigan Dalio
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado