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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phytophthora parasitica Effector PpRxLR2 Suppresses Nicotiana benthamiana Immunity

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Author(s):
Dalio, R. J. D. [1] ; Maximo, H. J. [1] ; Oliveira, T. S. [1] ; Dias, R. O. [2] ; Breton, M. C. [1] ; Felizatti, H. [3] ; Machado, M. [1]
Total Authors: 7
Affiliation:
[1] Ctr Citricultura Sylvio Moreira, Inst Agron, Biotechnol Lab, Cordeiropolis, SP - Brazil
[2] Univ Sao Paulo, Inst Quim, Sao Paulo, SP - Brazil
[3] Univ Estadual Campinas Unicamp, Inst Matemat Fis & Comp Cient, Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: MOLECULAR PLANT-MICROBE INTERACTIONS; v. 31, n. 4, p. 481-493, APR 2018.
Web of Science Citations: 5
Abstract

Phytophthora species secrete several classes of effector proteins during interaction with their hosts. These proteins can have multiple functions including modulation of host physiology and immunity. The RxLR effectors have the ability to enter plant cells using the plant machinery. Some of these effectors have been characterized as immunity suppressors; however, very little is known about their functions in the interaction between Phytophthora parasitica and its hosts. Using a bioinformatics pipeline, we have identified 172 candidate RxLR effectors (CREs) in the isolate IAC 01\_95 of P. parasitica. Of these 172 CREs, 93 were found to be also present in eight other genomes of P. parasitica, isolated from different hosts and continents. After transcriptomics and gene expression analysis, we have found five CREs to be up-regulated in in-vitro and in-planta samples. Subsequently, we selected three CREs for functional characterization in the model plant Nicotiana benthamiana. We show that PpRxLR2 is able to completely suppress INF-1-induced cell death, whereas PpRxLR3 and PpRxLR5 moderately suppressed N. benthamiana immunity in a less-extensive manner. Moreover, we confirmed the effector-triggered susceptibility activity of these proteins after transient transformation and infection of N. benthamiana plants. All three CREs enhanced virulence of P. parasitica during the interaction with N. benthamiana. These effectors, in particular PpRxLR2, can be targeted for the development of biotechnology-based control strategies of P. parasitica diseases. (AU)

FAPESP's process: 15/14498-6 - Effectoromics: effectors as a new tool to control Phytophthora parasitica, causal agent of citrus root rot and gummosis.
Grantee:Ronaldo José Durigan Dalio
Support Opportunities: Scholarships in Brazil - Post-Doctoral