In vitro maturation impacts cumulus-oocyte complex metabolism and stress in cattle

Autor(es):Mostrar menos -

del Collado, Maite ^[1] ; da Silveira, Juliano C. ^[1] ; Oliveira, Marcelo L. F. ^[1] ; Alves, Barbara M. S. M. ^[1] ; Simas, Rosineide C. ^[2] ; Godoy, Adriana T. ^[2] ; Coelho, Mirela B. ^[2] ; Marques, Lygia A. ^[2] ; Carriero, Mateus M. ^[1] ; Nogueira, Marcelo F. G. ^[3] ; Eberlin, Marcos N. ^[2] ; Silva, Luciano A. ^[1] ; Meirelles, Flavio V. ^[1] ; Perecin, Felipe ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn FZEA, Vet Med Dept, Pirassununga, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil

^[3] Sao Paulo State Univ, Sch Sci Humanities & Languages, Biol Sci Dept, Assis, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Reproduction; v. 154, n. 6, p. 881-893, DEC 2017.
• Citações Web of Science: 3

Resumo

The influence of in vitro maturation (IVM) in oocytes is still not totally understood. The aim of this study was to determine the influence of IVM on the metabolism and homeostasis of bovine cumulus-oocyte complexes. In the present study, we demonstrated that IVM leads to accumulation of neutral lipids associated with differential levels of the mono-, di-and triacylglycerols in both cumulus cells and oocytes. We observed that in vitro-matured oocytes exhibited decreased glutathione and reactive oxygen species levels and a lower ATP/ADP ratio when compared to in vivo-matured oocytes, with no significant differences in metabolism and stress-related mRNA or miRNA levels. Moreover, in addition to an increase in lipids in in vitro-matured cumulus cells, fatty acid synthesis and accumulation as well as glycolysis pathway genes were upregulated, whereas those affiliated with the beta-oxidation pathway were decreased. Our gene expression data in cumulus cells suggest the disruption of endoplasmic reticulum stress, apoptosis and cellular stress response pathways during IVM. Furthermore, a total of 19 miRNAs were significantly altered by the maturation process in cumulus cells. These results indicate some new negative influences of the in vitro system in cumulus-oocyte complexes, demonstrating the occurrence of functional disruption in lipid metabolism and stress pathways and showing evidences suggesting the occurrence of altered mitochondrial activity and energy metabolism during IVM, with a massive dysregulation of the corresponding transcripts in the surrounding cumulus cells. (AU)

• Processo FAPESP: 14/03281-3 - Dinâmica lipídico-mitocondrial e moduladores do metabolismo oxidativo em embriões bovinos produzidos in vitro
• Beneficiário: Maite Del Collado Barrondo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/50533-2 - GIFT: melhoramento genômico de características relacionadas com a fertilização em gado bovino dinamarquês e brasileiro
• Beneficiário: Marcelo Fábio Gouveia Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/22887-0 - Exossomos e microvesículas contendo miRNAs modulam mudanças epigenéticas durante o cultivo in vitro de gametas e embriões em bovinos
• Beneficiário: Juliano Coelho da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 14/21034-3 - Rotas intracelulares e intercelulares durante a foliculogênese e maturação oocitária in vivo e in vitro
• Beneficiário: Felipe Perecin
• Modalidade de apoio: Auxílio à Pesquisa - Regular