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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro maturation impacts cumulus-oocyte complex metabolism and stress in cattle

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Author(s):
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del Collado, Maite [1] ; da Silveira, Juliano C. [1] ; Oliveira, Marcelo L. F. [1] ; Alves, Barbara M. S. M. [1] ; Simas, Rosineide C. [2] ; Godoy, Adriana T. [2] ; Coelho, Mirela B. [2] ; Marques, Lygia A. [2] ; Carriero, Mateus M. [1] ; Nogueira, Marcelo F. G. [3] ; Eberlin, Marcos N. [2] ; Silva, Luciano A. [1] ; Meirelles, Flavio V. [1] ; Perecin, Felipe [1]
Total Authors: 14
Affiliation:
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn FZEA, Vet Med Dept, Pirassununga, SP - Brazil
[2] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil
[3] Sao Paulo State Univ, Sch Sci Humanities & Languages, Biol Sci Dept, Assis, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Reproduction; v. 154, n. 6, p. 881-893, DEC 2017.
Web of Science Citations: 3
Abstract

The influence of in vitro maturation (IVM) in oocytes is still not totally understood. The aim of this study was to determine the influence of IVM on the metabolism and homeostasis of bovine cumulus-oocyte complexes. In the present study, we demonstrated that IVM leads to accumulation of neutral lipids associated with differential levels of the mono-, di-and triacylglycerols in both cumulus cells and oocytes. We observed that in vitro-matured oocytes exhibited decreased glutathione and reactive oxygen species levels and a lower ATP/ADP ratio when compared to in vivo-matured oocytes, with no significant differences in metabolism and stress-related mRNA or miRNA levels. Moreover, in addition to an increase in lipids in in vitro-matured cumulus cells, fatty acid synthesis and accumulation as well as glycolysis pathway genes were upregulated, whereas those affiliated with the beta-oxidation pathway were decreased. Our gene expression data in cumulus cells suggest the disruption of endoplasmic reticulum stress, apoptosis and cellular stress response pathways during IVM. Furthermore, a total of 19 miRNAs were significantly altered by the maturation process in cumulus cells. These results indicate some new negative influences of the in vitro system in cumulus-oocyte complexes, demonstrating the occurrence of functional disruption in lipid metabolism and stress pathways and showing evidences suggesting the occurrence of altered mitochondrial activity and energy metabolism during IVM, with a massive dysregulation of the corresponding transcripts in the surrounding cumulus cells. (AU)

FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 14/21034-3 - Intra and intercellular routes during foliculogenesis and in vivo and in vitro oocyte maturation
Grantee:Felipe Perecin
Support type: Regular Research Grants
FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
Grantee:Marcelo Fábio Gouveia Nogueira
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/03281-3 - Lipid-mitochondrial dynamic and oxidative metabolism modulators in in vitro produced bovine embryos
Grantee:Maite Del Collado Barrondo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC