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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

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Autor(es):
Kague, E. [1, 2] ; Witten, P. E. [3] ; Soenens, M. [3] ; Campos, C. L. [1] ; Lubiana, T. [1] ; Fisher, S. [4] ; Hammond, C. [2] ; Brown, K. Robson [5] ; Passos-Bueno, M. R. [1] ; Huysseune, A. [3]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, Ctr Pesquisa Genoma Humano & Celulas Tronco, Sao Paulo - Brazil
[2] Univ Bristol, Dept Physiol Pharmacol & Neurosci, Bristol BS8 1TD, Avon - England
[3] Univ Ghent, Evolutionary Dev Biol, Ghent - Belgium
[4] Boston Univ, Sch Med, Dept Pharmacol & Expt Therapeut, Boston, MA 02215 - USA
[5] Univ Bristol, Sch Archaeol & Anthropol, Bristol, Avon - England
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Developmental Biology; v. 435, n. 2, p. 176-184, MAR 15 2018.
Citações Web of Science: 2
Resumo

The capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteodast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised. (AU)

Processo FAPESP: 13/08028-1 - CEGH-CEL - Centro de Estudos do Genoma Humano e de Células-Tronco
Beneficiário:Mayana Zatz
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs