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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

Full text
Author(s):
Kague, E. [1, 2] ; Witten, P. E. [3] ; Soenens, M. [3] ; Campos, C. L. [1] ; Lubiana, T. [1] ; Fisher, S. [4] ; Hammond, C. [2] ; Brown, K. Robson [5] ; Passos-Bueno, M. R. [1] ; Huysseune, A. [3]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, Ctr Pesquisa Genoma Humano & Celulas Tronco, Sao Paulo - Brazil
[2] Univ Bristol, Dept Physiol Pharmacol & Neurosci, Bristol BS8 1TD, Avon - England
[3] Univ Ghent, Evolutionary Dev Biol, Ghent - Belgium
[4] Boston Univ, Sch Med, Dept Pharmacol & Expt Therapeut, Boston, MA 02215 - USA
[5] Univ Bristol, Sch Archaeol & Anthropol, Bristol, Avon - England
Total Affiliations: 5
Document type: Journal article
Source: Developmental Biology; v. 435, n. 2, p. 176-184, MAR 15 2018.
Web of Science Citations: 4
Abstract

The capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteodast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised. (AU)

FAPESP's process: 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center
Grantee:Mayana Zatz
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC