Human papillomavirus detection using PCR and ATR-FTIR for cervical cancer screening

Autor(es)

Rymsza, Taciana ^[1] ; Ribeiro, Eliane Aline ^[1] ; das Chagas e Silva de Carvalho, Luis Felipe ^{[2, 3]} ; Bhattacharjee, Tanmoy ^[4] ; Canevari, Renata de Azevedo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Lab Biol Mol Canc, UNIVAP, Inst Pesquisa & Desenvolvimento, Ave Shishima Hifumi 2911, BR-12244000 Sao Paulo, SP - Brazil

^[2] Univ Vale Paraiba, Lab Espect Vibrac Biomed, UNIVAP, Inst Pesquisa & Desenvolvimento, Ave Shishima Hifumi 2911, BR-12244000 Sao Paulo, SP - Brazil

^[3] Univ Taubate, Dept Odontol, UNITAU, Rua Operarios 53, BR-12020270 Sao Paulo, SP - Brazil

^[4] Univ Vale Paraiba, Lab Nanosensores, UNIVAP, Inst Pesquisa & Desenvolvimento, Ave Shishima Hifumi 2911, BR-12244000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 196, p. 238-246, MAY 5 2018.
• Citações Web of Science: 3

Resumo

The human papillomavirus (HPV) genital infection is considered one of the most common sexually transmitted diseases worldwide, and has been associated with cervical cancer. The objective of this study was to investigate the efficacy of the diagnostic methods: polymerase chain reaction (PCR) and Fourier transform infrared (FTIR) equipped with an ATR (Attenuated Total Reflectance) unit (Pike Tech) spectroscopy, to diagnose HPV infection in women undergoing gynecological examination. Seventeen patients (41.46%) of the 41 patients analyzed were diagnosed with exophytic/condyloma acuminate lesions by clinical analysis, 29 patients (70.7%) (G1 group) of the 41 patients, showed positive result for HPV cell injury by oncotic colpocitology and 12 patients (293%) (G2 group), presented negative result for cellular lesion and absence of clinical HPV lesion. Four samples were obtained per patient, which were submitted oncotic colpocitology analysis (Papanicolau staining, two samples), PCR (one sample) and ATR-FTIR analysis (one sample). L1 gene was amplified by PCR technique with specific GP5+/GP6+ and MY09/MY11 primers. PCR results were uniformly positive for presence of HPV in all analyzed samples. Multivariate analysis of ATR-FTIR spectra suggests no significant biochemical changes between groups and no clustering formed, concurring with results of PCR. This study suggests that PCR and ATR-FTIR are highly sensitive technique for HPV detection. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/14464-9 - Caracterização molecular e bioquímica de lesões da tireóide para diagnóstico clínico
• Beneficiário: Renata de Azevedo Canevari
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/18440-1 - Desenvolvimento de marcadores moleculares relacionados à resistência celular após terapia fotodinâmica em carcinomas mamários
• Beneficiário: Renata de Azevedo Canevari
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/05978-1 - Utilização da espectroscopia Raman in vivo para diagnóstico de processos patológicos bucais
• Beneficiário: Luis Felipe das Chagas e Silva de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado