Association study between copy number variation and beef fatty acid profile of Nellore cattle

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de Lemos, Marcos Vinicius Antunes ^[1] ; Peripolli, Elisa ^[1] ; Berton, Mariana Piatto ^[1] ; Braga Feitosa, Fabiele Loise ^[1] ; Olivieri, Bianca Ferreira ^[1] ; Stafuzza, Nedenia Bonvino ^[1] ; Tonussi, Rafael Lara ^[1] ; Kluska, Sabrina ^[1] ; Justino Chiaia, Hermenegildo Lucas ^[1] ; Mueller, Lenise ^[2] ; Ferrinho, Adrielli Mathias ^[2] ; Cravo Prereira, Angelica Simone ^[2] ; de Oliveira, Henrique Nunes ^[1] ; de Albuquerque, Lucia Galvo ^[1] ; Baldi, Fernando ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Fac Ciencias Agr & Vet, BR-14884000 Jaboticabal, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, BR-13635900 Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF APPLIED GENETICS; v. 59, n. 2, p. 203-223, MAY 2018.
• Citações Web of Science: 5

Resumo

The aim of this study was to analyze the association between the copy number variation regions (CNVRs) and fatty acid profile phenotypes for saturated (SFA), monosaturated (MUFA), polyunsaturated (PUFA), omega 6 and omega 3 fatty acids, PUFA/SFA and omega 6/omega 3 ratios, as well as for their sums, in Nellore cattle (Bos primigenius indicus). A total of 963 males were finished in feedlot and slaughtered with approximately 2 years of age. Animals were genotyped with the BovineHD BeadChip (Illumina Inc., San Diego, CA, USA). The copy number variation (CNV) detection was performed using the PennCNV algorithm. Log R ratio (LRR) and allele B frequency (BAF) were used to estimate the CNVs. The association analyses were done using the CNVRuler software and applying a logistic regression model. The phenotype was adjusted using a linear model considering the fixed effects of contemporary group and the animal age at slaughter. The fatty acid profile was analyzed on samples of longissimus thoracis muscle using gas chromatography with a 100-m capillary column. For the association analysis, the adjusted phenotypic values were considered for the traits, while the data was adjusted for the effects of the farm and year of birth, management groups at birth, weaning, and superannuation. A total of 186 CNVRs were significant for SFA (43), MUFA (42), PUFA (66), and omega fatty acid (35) groups, totaling 278 known genes. On the basis of the results, several genes were associated with several fatty acids of different saturations. Olfactory receptor genes were associated with C12:0, C14:0, and C18:0 fatty acids. The SAMD8 and BSCL2 genes, both related to lipid metabolic process, were associated with C12:0. The RAPGEF6 gene was found to be associated with C18:2 cis-9 cis-12 n-6, and its function is related to regulation of GTPase activity. Among the results, we highlighted the olfactory receptor activity (GO:0004984), G-protein-coupled receptor activity (GO:0004930), potassium:proton antiporter activity (GO:0015386), sodium:proton antiporter activity (GO:0015385), and odorant-binding (GO:0005549) molecular functions. A large number of genes associated with fatty acid profile within the CNVRs were identified in this study. These findings must contribute to better elucidate the genetic mechanism underlying the fatty acid profile of intramuscular fat in Nellore cattle. (AU)

• Processo FAPESP: 09/16118-5 - Ferramentas genômicas no melhoramento genético de características de importância econômica direta em bovinos da raça Nelore
• Beneficiário: Lucia Galvão de Albuquerque
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/11853-4 - Análise das variações no número de cópias no genoma de bovinos Nelore e suas associações com o perfil de ácidos graxos da carne
• Beneficiário: Marcos Vinícius Antunes de Lemos
• Modalidade de apoio: Bolsas no Brasil - Doutorado