The Zinc-Finger Thylakoid-Membrane Protein FIP Is Involved With Abiotic Stress Response in Arabidopsis thaliana

Autor(es)

Lopes, Karina L. ^[1] ; Rodrigues, Ricardo A. O. ^[1] ; Silva, Marcos C. ^[1] ; Braga, Wiliane G. S. ^[1] ; Silva-Filho, Marcio C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 9, APR 18 2018.
• Citações Web of Science: 0

Resumo

Many plant genes have their expression modulated by stress conditions. Here, we used Arabidopsis FtsH5 protease, which expression is regulated by light stress, as bait in a yeast two-hybrid screen to search for new proteins involved in the stress response. As a result, we found FIP (FtsH5 Interacting Protein), which possesses an amino proximal cleavable transit peptide, a hydrophobic membrane-anchoring region, and a carboxyl proximal C-4-type zinc-finger domain. In vivo experiments using FIP fused to green fluorescent protein (GFP) showed a plastid localization. This finding was corroborated by chloroplast import assays that showed FIP inserted in the thylakoid membrane. FIP expression was down-regulated in plants exposed to high light intensity, oxidative, salt, and osmotic stresses, whereas mutant plants expressing low levels of FIP were more tolerant to these abiotic stresses. Our data shows a new thylakoid-membrane protein involved with abiotic stress response in Arabidopsis thaliana. (AU)

• Processo FAPESP: 14/50275-9 - Decifrando os mecanismos moleculares envolvidos na localização subcelular de proteínas e na complexa interação planta-insetos-patógenos
• Beneficiário: Márcio de Castro Silva Filho
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 14/16034-4 - Caracterização funcional da FIP (FtsH5 interacting protein): um possível regulador da atividade da protease FtsH5 de Arabidopsis thaliana
• Beneficiário: Karina Letícia Lopes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/57806-6 - Decifrando os mecanismos moleculares envolvidos na localização de membros da família das FtsHs plastidiais nas membranas dos tilacóides
• Beneficiário: Ricardo Augusto de Oliveira Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Doutorado