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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Zinc-Finger Thylakoid-Membrane Protein FIP Is Involved With Abiotic Stress Response in Arabidopsis thaliana

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Lopes, Karina L. [1] ; Rodrigues, Ricardo A. O. [1] ; Silva, Marcos C. [1] ; Braga, Wiliane G. S. [1] ; Silva-Filho, Marcio C. [1]
Total Authors: 5
[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba - Brazil
Total Affiliations: 1
Document type: Journal article
Source: FRONTIERS IN PLANT SCIENCE; v. 9, APR 18 2018.
Web of Science Citations: 0

Many plant genes have their expression modulated by stress conditions. Here, we used Arabidopsis FtsH5 protease, which expression is regulated by light stress, as bait in a yeast two-hybrid screen to search for new proteins involved in the stress response. As a result, we found FIP (FtsH5 Interacting Protein), which possesses an amino proximal cleavable transit peptide, a hydrophobic membrane-anchoring region, and a carboxyl proximal C-4-type zinc-finger domain. In vivo experiments using FIP fused to green fluorescent protein (GFP) showed a plastid localization. This finding was corroborated by chloroplast import assays that showed FIP inserted in the thylakoid membrane. FIP expression was down-regulated in plants exposed to high light intensity, oxidative, salt, and osmotic stresses, whereas mutant plants expressing low levels of FIP were more tolerant to these abiotic stresses. Our data shows a new thylakoid-membrane protein involved with abiotic stress response in Arabidopsis thaliana. (AU)

FAPESP's process: 14/50275-9 - Deciphering the molecular mechanisms involved in the localization of organelar proteins as well as the complex plant-insect-pathogen interactions
Grantee:Márcio de Castro Silva Filho
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 14/16034-4 - Functional characterization of FIP (FtsH5 interacting protein): a potential regulator of the FtsH5 protease activity in Arabidopsis thaliana
Grantee:Karina Letícia Lopes
Support type: Scholarships in Brazil - Doctorate