Licochalcone A induces morphological and biochemical alterations in Schistosoma mansoni adult worms

Autor(es):Mostrar menos -

Souza, Ritieres Lovo ^[1] ; Goncalves, Ubirajara Oliveira ^[1] ; Badoco, Fernanda Rafacho ^[1] ; Galvao, Lucas de Souza ^[1] ; dos Santos, Raquel Alves ^[1] ; Dias de Carvalho, Paulo Henrique ^[2] ; Aleixo de Carvalho, Lara Soares ^[2] ; da Silva Filho, Ademar Alves ^[2] ; Sola Veneziani, Rodrigo Cassio ^[1] ; Rodrigues, Vanderlei ^[3] ; Ambrosio, Sergio Ricardo ^[1] ; Magalhaes, Lizandra Guidi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, Res Grp Nat Prod, Ctr Res Sci & Technol, Franca, SP - Brazil

^[2] Univ Fed Juiz de Fora, Coll Pharm, Dept Pharmaceut Sci, Juiz De Fora, MG - Brazil

^[3] Univ Sao Paulo, Coll Med Ribeirao Preto, Dept Biochem & Immunol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICINE & PHARMACOTHERAPY; v. 96, p. 64-71, DEC 2017.
• Citações Web of Science: 2

Resumo

This paper is the first report on the in vitro effects of licochalcone A, a chalcone isolated from Glycyrrhiza inflate Batalin (Leguminosae), on Schistosoma mansoni adult worms. In vitro, licochalcone A afforded lethal concentrations for 50% of parasites (LC50) of 9.12 +/- 1.1 and 9.52 +/- 0.9 mu M against female and male adult worms, respectively, at 24 h. Additionally, the compound reduced the total number of S. mansoni eggs and affected the development of eggs produced by S. mansoni adult worms. Together, the results achieved after 24 h showed that licochalcone A was 55.7- and 53.3-fold more toxic to S. mansoni female and male adult worms than to Chinese hamster ovary fibroblasts cells, respectively. Treatment with licochalcone A elicited drastic changes in the tegument of S. mansoni adult worms, as well as mitochondrial alteration and chromatin condensation. Licochalcone A also increased the superoxide anion level and decreased the superoxide dismutase activity in S. mansoni adult worms. Overall, our results indicated that licochalcone A displays in vitro schistosomicidal activity. This effect may result from increased production of reactive oxygen species (ROS) induced by the action of licochalcone A. The resulting ROS could act on the S. mansoni tegument and membranes and help induce the death of S. mansoni adult worms. (AU)

• Processo FAPESP: 13/11164-4 - Avaliação da indução da apoptose em vermes adultos de Schistosoma mansoni após exposição com curcumina
• Beneficiário: Lizandra Guidi Magalhães
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/24456-1 - Curcuminóides monocetônicos para o tratamento da esquistossomose: uma avaliação pré-clínica da atividade esquistossomicida
• Beneficiário: Lizandra Guidi Magalhães
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/01394-8 - Estudo do análogo de curcumina EF-24 e praziquantel nos aspectos bioquímicos e morfológicos envolvidos com a morte celular do parasito Schistosoma mansoni
• Beneficiário: Fernanda Rafacho Badoco
• Linha de fomento: Bolsas no Brasil - Mestrado