Licochalcone A induces morphological and biochemical alterations in Schistosoma mansoni adult worms

Author(s):Show less -

Souza, Ritieres Lovo ^[1] ; Goncalves, Ubirajara Oliveira ^[1] ; Badoco, Fernanda Rafacho ^[1] ; Galvao, Lucas de Souza ^[1] ; dos Santos, Raquel Alves ^[1] ; Dias de Carvalho, Paulo Henrique ^[2] ; Aleixo de Carvalho, Lara Soares ^[2] ; da Silva Filho, Ademar Alves ^[2] ; Sola Veneziani, Rodrigo Cassio ^[1] ; Rodrigues, Vanderlei ^[3] ; Ambrosio, Sergio Ricardo ^[1] ; Magalhaes, Lizandra Guidi ^[1]

Affiliation

^[1] Univ Franca, Res Grp Nat Prod, Ctr Res Sci & Technol, Franca, SP - Brazil

^[2] Univ Fed Juiz de Fora, Coll Pharm, Dept Pharmaceut Sci, Juiz De Fora, MG - Brazil

^[3] Univ Sao Paulo, Coll Med Ribeirao Preto, Dept Biochem & Immunol, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: BIOMEDICINE & PHARMACOTHERAPY; v. 96, p. 64-71, DEC 2017.
• Web of Science Citations: 2

Abstract

This paper is the first report on the in vitro effects of licochalcone A, a chalcone isolated from Glycyrrhiza inflate Batalin (Leguminosae), on Schistosoma mansoni adult worms. In vitro, licochalcone A afforded lethal concentrations for 50% of parasites (LC50) of 9.12 +/- 1.1 and 9.52 +/- 0.9 mu M against female and male adult worms, respectively, at 24 h. Additionally, the compound reduced the total number of S. mansoni eggs and affected the development of eggs produced by S. mansoni adult worms. Together, the results achieved after 24 h showed that licochalcone A was 55.7- and 53.3-fold more toxic to S. mansoni female and male adult worms than to Chinese hamster ovary fibroblasts cells, respectively. Treatment with licochalcone A elicited drastic changes in the tegument of S. mansoni adult worms, as well as mitochondrial alteration and chromatin condensation. Licochalcone A also increased the superoxide anion level and decreased the superoxide dismutase activity in S. mansoni adult worms. Overall, our results indicated that licochalcone A displays in vitro schistosomicidal activity. This effect may result from increased production of reactive oxygen species (ROS) induced by the action of licochalcone A. The resulting ROS could act on the S. mansoni tegument and membranes and help induce the death of S. mansoni adult worms. (AU)

• FAPESP's process: 15/01394-8 - Study of EF-24 curcumin analogue and praziquantel in biochemical and morphological aspects involved in the cell death of the Schistosoma mansoni parasite
• Grantee: Fernanda Rafacho Badoco
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/24456-1 - Monoketo enone curcuminoids the treatment of schistosomiasis: an pre-clinical evaluation of schistosomicidal activity
• Grantee: Lizandra Guidi Magalhães
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/11164-4 - Evaluation of apoptosis induction on Schistosoma mansoni adult worm after exposure with curcumin
• Grantee: Lizandra Guidi Magalhães
• Support Opportunities: Regular Research Grants