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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Mast Cell Mediators Inhibit Osteoblastic Differentiation and Extracellular Matrix Mineralization

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Autor(es):
Amaru Maximiano, William Marcatti [1] ; Marcelino da Silva, Elaine Zayas [1] ; Santana, Ana Carolina [1] ; de Oliveira, Paulo Tambasco [2] ; Jamur, Maria Celia [1] ; Oliver, Constance [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol & Pathogen Bioagents, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Morphol Stomatol & Basic Pathol, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY; v. 65, n. 12, p. 723-741, DEC 2017.
Citações Web of Science: 2
Resumo

Mast cells are multifunctional immune cells that participate in many important processes such as defense against pathogens, allergic reactions, and tissue repair. These cells perform their functions through the release of a wide variety of mediators. This release occurs mainly through cross-linking IgE (immunoglobulin E) bound to high affinity IgE receptors by multivalent antigens. The abundance of mast cells in connective tissue, surrounding blood vessels, and their involvement in the early stages of bone repair support the possibility of physiological and pathological interactions between mast cells and osteoblasts. However, the participation of mast cell mediators in osteogenesis is not fully understood. Therefore, the objective of this work was to investigate the role of mast cell mediators in the acquisition of the osteogenic phenotype in vitro. The results show that pooled mast cell mediators can affect proliferation, morphology, and cytoskeleton of osteoblastic cells, and impair the activity and expression of alkaline phosphatase as well as the expression of bone sialoprotein. Also, mast cell mediators inhibit the expression of mRNA for those proteins and inhibit the formation and maturation of calcium nodules and consequently inhibit mineralization. Therefore, mast cell mediators can modulate osteogenesis and are potential therapeutic targets for treatments of bone disorders. (AU)

Processo FAPESP: 10/51850-6 - Caracterização do papel dos mastócitos na osteogênese
Beneficiário:William Marcatti Amaru Maximiano
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 09/54013-0 - Correlação funcional entre mastócitos e a angiogênese tumoral
Beneficiário:Maria Célia Jamur
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 12/06373-0 - O envolvimento do complexo adaptador AP-3 na secreção regulada em mastócitos
Beneficiário:Elaine Zayas Marcelino da Silva
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 16/50298-4 - Funcionalização de superfície de materiais bioativos com moléculas naturais para a modulação da resposta tecidual a implantes aloplásticos
Beneficiário:Paulo Tambasco de Oliveira
Linha de fomento: Auxílio à Pesquisa - Regular