Mast Cell Mediators Inhibit Osteoblastic Differentiation and Extracellular Matrix Mineralization

Author(s)

Amaru Maximiano, William Marcatti ^[1] ; Marcelino da Silva, Elaine Zayas ^[1] ; Santana, Ana Carolina ^[1] ; de Oliveira, Paulo Tambasco ^[2] ; Jamur, Maria Celia ^[1] ; Oliver, Constance ^[1]

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol & Pathogen Bioagents, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Morphol Stomatol & Basic Pathol, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY; v. 65, n. 12, p. 723-741, DEC 2017.
• Web of Science Citations: 2

Abstract

Mast cells are multifunctional immune cells that participate in many important processes such as defense against pathogens, allergic reactions, and tissue repair. These cells perform their functions through the release of a wide variety of mediators. This release occurs mainly through cross-linking IgE (immunoglobulin E) bound to high affinity IgE receptors by multivalent antigens. The abundance of mast cells in connective tissue, surrounding blood vessels, and their involvement in the early stages of bone repair support the possibility of physiological and pathological interactions between mast cells and osteoblasts. However, the participation of mast cell mediators in osteogenesis is not fully understood. Therefore, the objective of this work was to investigate the role of mast cell mediators in the acquisition of the osteogenic phenotype in vitro. The results show that pooled mast cell mediators can affect proliferation, morphology, and cytoskeleton of osteoblastic cells, and impair the activity and expression of alkaline phosphatase as well as the expression of bone sialoprotein. Also, mast cell mediators inhibit the expression of mRNA for those proteins and inhibit the formation and maturation of calcium nodules and consequently inhibit mineralization. Therefore, mast cell mediators can modulate osteogenesis and are potential therapeutic targets for treatments of bone disorders. (AU)

• FAPESP's process: 12/06373-0 - The involvement of the adaptor protein complex AP-3 in mast cell regulated secretion
• Grantee: Elaine Zayas Marcelino da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/50298-4 - Natural molecules on the surface of bioactive materials for modulating the host response to implants (NAT4MORE)
• Grantee: Paulo Tambasco de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/54013-0 - Functional correlation between mast cells and tumor angiogenesis
• Grantee: Maria Célia Jamur
• Support Opportunities: Multi-user Equipment Program