Porous Titanium Associated with CaP Coating: In Vivo and In Vitro Osteogenic Performance

Autor(es)

Luana Marotta Reis de Vasconcellos ^[1] ; Rodrigo Dias Nascimento ^[2] ; Carlos Alberto Alves Cairo ^[3] ; Daniel de Oliveira Leite ^[1] ; Evelyn Luzia de Souza Santos ^[1] ; Gabriela Esteves Campos ^[1] ; Renata Falchete do Prado ^[1] ; Maria Aparecida Neves Jardini ^[2] ; Luis Gustavo Oliveira de Vasconcellos ^[4] ; Yasmin Rodarte Carvalho ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Dept Biociencias & Diagnost Oral, Inst Ciencia & Tecnol Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Dept Diagnost & Cirurgia, Inst Ciencia & Tecnol Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil

^[3] CTA, Inst Divisao Mat Are & Espaco, Sao Jose Dos Campos, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Dept Mat Odontol & Protese, Inst Ciencia & Tecnol Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS; v. 21, n. 2 2017-12-18.

Resumo

This in vitro and in vivo study compared different topographies of Ti samples (dense, porosity of 30% and 40%) with or not CaP coating, prepared by powder metallurgy. Osteogenic cells from newborn rat calvaria were plated onto the samples and cell adhesion (24 hours), alkaline phosphatase activity (7 and 10 days) and mineralization nodules (14 days) were assessed. Sixteen rabbits were used for in vivo study. Each animal received three non-treated and three treated implants in the right or left tibia, respectively. Histometric evaluation of bone-implant contact (BIC) were assessed at 1, 2, 4 and 8 weeks. Metallographic analysis revealed porosities of 30% and 40%, with pore size ranging from 250 to 350 µm. Cell adhesion test and ALP revealed similar cell behavior, independently of topography and CaP coating (P > 0.05%). However, CaP coating combined with porosity of 40% influenced positively the mineralized matrix formation (P < 0.05%). CaP-coated implants showed higher BIC than non-CaP implants and BIC was different between the short (1 and 2 weeks) and long (4 and 8 weeks) healing periods (P < 0.05%). The results suggest that CaP coating combined with 40% porosity implants allowed greater osteogenesis in vitro and increased BIC in vivo. (AU)

• Processo FAPESP: 05/03709-4 - Estudo da osseointegração de implantes porosos: análise do crescimento ósseo nos poros, resistência da interface osso-implante e da propriedade mecânica do titânio poroso
• Beneficiário: Luana Marotta Reis de Vasconcellos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/08593-0 - Osteogênese in vitro e in vivo em amostras de titânio, com diferentes porosidades, submetidas ao tratamento biomimético
• Beneficiário: Yasmin Rodarte Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular