Porous Titanium Associated with CaP Coating: In Vivo and In Vitro Osteogenic Performance

Author(s)

Luana Marotta Reis de Vasconcellos ^[1] ; Rodrigo Dias Nascimento ^[2] ; Carlos Alberto Alves Cairo ^[3] ; Daniel de Oliveira Leite ^[1] ; Evelyn Luzia de Souza Santos ^[1] ; Gabriela Esteves Campos ^[1] ; Renata Falchete do Prado ^[1] ; Maria Aparecida Neves Jardini ^[2] ; Luis Gustavo Oliveira de Vasconcellos ^[4] ; Yasmin Rodarte Carvalho ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Dept Biociencias & Diagnost Oral, Inst Ciencia & Tecnol Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Dept Diagnost & Cirurgia, Inst Ciencia & Tecnol Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil

^[3] CTA, Inst Divisao Mat Are & Espaco, Sao Jose Dos Campos, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Dept Mat Odontol & Protese, Inst Ciencia & Tecnol Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS; v. 21, n. 2 2017-12-18.

Abstract

This in vitro and in vivo study compared different topographies of Ti samples (dense, porosity of 30% and 40%) with or not CaP coating, prepared by powder metallurgy. Osteogenic cells from newborn rat calvaria were plated onto the samples and cell adhesion (24 hours), alkaline phosphatase activity (7 and 10 days) and mineralization nodules (14 days) were assessed. Sixteen rabbits were used for in vivo study. Each animal received three non-treated and three treated implants in the right or left tibia, respectively. Histometric evaluation of bone-implant contact (BIC) were assessed at 1, 2, 4 and 8 weeks. Metallographic analysis revealed porosities of 30% and 40%, with pore size ranging from 250 to 350 µm. Cell adhesion test and ALP revealed similar cell behavior, independently of topography and CaP coating (P > 0.05%). However, CaP coating combined with porosity of 40% influenced positively the mineralized matrix formation (P < 0.05%). CaP-coated implants showed higher BIC than non-CaP implants and BIC was different between the short (1 and 2 weeks) and long (4 and 8 weeks) healing periods (P < 0.05%). The results suggest that CaP coating combined with 40% porosity implants allowed greater osteogenesis in vitro and increased BIC in vivo. (AU)

• FAPESP's process: 05/03709-4 - Study of porous implants osseointegration: analysis of bone growth in pores, resistance of implant-bone interface and mechanical property of porous titanium
• Grantee: Luana Marotta Reis de Vasconcellos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 07/08593-0 - In vitro and in vivo osteogenesis on titanium samples, with different porosities, submitted to biomimetic treatment
• Grantee: Yasmin Rodarte Carvalho
• Support Opportunities: Regular Research Grants