3D coating of nanobiosilica nanobiosilica coating extracted from marine sponges to improve osseointegration of titanium implants.

• Grant number: 23/10900-0
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: January 29, 2024
• End date: July 28, 2024
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Ana Claudia Muniz Renno
• Grantee: Giovanna do Espirito Santo
• Supervisor: Silvia Marcela Cere
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Institution abroad: Universidad Nacional de Mar del Plata (UNMdP), Argentina
• Associated to the scholarship: 22/04433-8 - Manufacture of 3D marine sponge from biosilica scaffolds for use in bone tissue engineering and their evaluation of biological effects in vitro, BP.MS

Abstract

Bone fractures impose a significant financial burden on healthcare systems in Latin America, including Brazil, where treatment of complex fractures often requires titanium implants due to their biocompatibility and mechanical stability. However, to improve osseointegration and reduce corrosion of titanium, proper surface treatment is essential. In search of convenient alternatives similar to nanobioceramics, the marine environment offers options with bioactive and biocompatible properties. Among them, marine sponges have biosilica (BS) in their simple, porous skeleton, which has been shown to promote bone formation in in vivo and in vitro studies. Furthermore, it is essential to work with nanoscale morphology, as this significantly improves bioactivity and biocompatibility. The use of biosilica nanoparticles (nBS) is of utmost importance due to its biological effects and in addition, there are studies that indicate the possibility of covering a polymer layer using FDM 3D printing from a polymer matrix that incorporates nanobioceramics. With this in mind, it is hypothesized that the combination of these factors will stimulate osseointegration of the titanium implants compared to the 3D nBG coating group. Its objective is to print a 3D nBS coating on a titanium implant and compare its physicochemical, morphological characteristics and biological effects in vitro with the group of 3D nBS coatings. To evaluate the results, various tests will be carried out, such as electron microscopy, infrared spectroscopy, contact angle and roughness analysis. Additionally, the degradation behavior and corrosion resistance of artificial saliva will be examined, as well as in vitro tests to evaluate the rate of hemolysis and platelet adhesion. Statistical analysis will be performed using two-way ANOVA and Tukey's POST-HOC test, with a significance threshold of p<0.05.