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Antibacterial coating with photocatalytic potential under visible light for dental implants: in vitro, in situ and in vivo studies

Grant number: 19/17238-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2021
Effective date (End): February 29, 2024
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Valentim Adelino Ricardo Barão
Grantee:Bruna Egumi Nagay
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


To confirm the promising photocatalytic antibacterial and cytocompatibility effects of a coating developed in our previous study (Nagay et al. 2019) for dental implants application, the aim of the present study is to evaluate in vitro, in situ and in vivo the antibacterial, immunogenic and osteogenic activity of the TiO2 coating codoped with nitrogen (N) and bismuth (Bi) via Plasma Electrolytic Oxidation (PEO) of commercially pure titanium surface (cpTi), in the presence and absence of visible light. CpTi discs (10 mm in diameter × 1 mm thick) and implants (1.6 mm in diameter × 3 mm in height) will be subjected to different surface modifications: sandblasting with aluminum oxide followed by acid etching (control); and modified by PEO with surface of TiO2 coating codoped with N and Bi (Bi-TiO2, experimental). Three experiments will be conducted: (1) in vitro study, to evaluate the release of metal ions, the antimicrobial potential (microcosm model considering saliva of volunteers as inoculum) after application or not of visible light, and biocompatibility (pre-osteoblastic cells) of the coating developed on the discs; (2) in situ study, where the volunteers will use for 7 days a palatal appliance containing 3 control discs and 3 experimental discs, to allow the accumulation of biofilm, which will be irradiated by visible light or not (dark). Colony-Forming Unit (CFU) count and biofilm analysis by CLSM will be performed immediately and after 24 h of light application; (3) in vivo studies, to verify the osseointegration potential of PEO-treated implants containing Bi-TiO2 on the surface compared to control after implantation in rats tibiae. Histological and immunohistochemical analyzes for decalcified tissues, and micro Computed Tomography (micro-CT), bone-implant contact, newly formed bone area, reverse torque and osteogenic gene expression for calcified tissues will be performed. To verify the in vivo antibacterial effectiveness and inflammatory response of the surface treatment with and without the light application, discs (5 mm in diameter × 1 mm in height) with control and experimental surfaces will be implanted on dorsal region of rats. After in situ biofilm formation on the surfaces, 1 disc (control or experimental surfaces, with or without previous light application) will be installed in each animal. Colony-Forming Unit (CFU) count and cytokine analysis (IL-1 beta and TNF-alpha) will be performed. Quantitative data will be submitted to the most appropriate statistical analysis with 5% significance level. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NAGAY, BRUNA EGUMI; DINI, CAROLINE; BORGES, GUILHERME ALMEIDA; MESQUITA, MARCELO FERRAZ; CAVALCANTI, YURI WANDERLEY; MAGNO, MARCELA BARAUNA; MAIA, LUCIANNE COPLE; RICARDO BARAO, VALENTIM ADELINO. Clinical efficacy of anodized dental implants for implant-supported prostheses after different loading protocols: A systematic review and meta-analysis. Clinical Oral Implants Research, v. 32, n. 9, . (20/05231-4, 19/17238-6)
DUTRA, TAMIRES PEREIRA; SACRAMENTO, CATHARINA MARQUES; NAGAY, BRUNA EGUMI; MAGNO, MARCELA BARAUNA; MARANON-VASQUEZ, GUIDO ARTEMIO; MAIA, LUCIANNE COPLE; SALLUM, ENILSON ANTONIO; SILVERIO RUIZ, KARINA GONZALES. o smokers have a different gingival crevicular fluid cytokine/chemokine profile than nonsmokers in clinically healthy periodontal sites? A systematic review and meta-analysi. CLINICAL ORAL INVESTIGATIONS, v. 26, n. 2, . (19/01522-7, 19/17238-6)

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