The objective of this study is to evaluate the influence of porosity and pore size, and the biomimetic treatment in osteogenesis in vitro and in vivo in specimes of titanium. 180 specimes of pure titanium grade 2 will be made by powder metallurgy, which will be divided into six groups: a) Group 1: control-titanium smooth; b) Group 2: 30% porosity and pore with 300µm; c) Group 3: 40% porosity and pore with 300µm; d) Group 4: samples of the G1 submitted to biomimetic treatment (BT); e) Group 5: samples of the G2 submitted to the BT ; f) Group 6: samples of the G3 submitted to BT. Initially, the surface of 05 specimens of each group will be characterized by means of metallographic analysis, to determine the quantity, area, pore morphology and interconnection. Five specimens with BT, will also be submitted to the dispersion of energy spectrometry (EDS), and Raman spectroscopy to identify the chemical elements present. At this stage in vitro study, cells osteogenics obtained from calvarial of newborn rats will be cultived on 15 samples of each group and evaluated on adesion after 24 hours, proliferation after 3, 7 and 10 days and the viability cell will be detected by the Trypan blue. It will be also measured the contents of total protein and the activity of alkaline phosphatase to 4, 7, 14 and 21 days and the quantification of nodular formations of mineralized matrix will be make at 7, 10, 14 days using the red of Alizarina S 2%. The cellular morphology of actin cytoskeleton, and the core will be observed by direct fluorescence. In the stage in vivo, will be used 15 rabbits to assess the repair of bone tissue around the titanium implants by means histological and histomorphometric analysis. Each rabbit will receive an implant of each group, and the implants of G1, G2, G3 and G4 will be inserted in the right tibia and G5, G6, G7 and G8 in the left tibia. Five rabbits, randomly selected, will be sacrificed 04, 08 and 12 weeks after surgery. Metallographic analysis of the data will be submitted to the Kruskal-Wallis test and data cell culture experiments will be compare using the nonparametric Mann-Whitney and t-Student test. The analysis histomorphometric will be assess by ANOVA statistical, and Tukey test (p <0.05). (AU)
Articles published in Agência FAPESP Newsletter about the research grant:
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
REIS DE VASCONCELLOS, LUANA MAROTTA;
NASCIMENTO, RODRIGO DIAS;
ALVES CAIRO, CARLOS ALBERTO;
LEITE, DANIEL DE OLIVEIRA;
DE SOUZA SANTOS, EVELYN LUZIA;
CAMPOS, GABRIELA ESTEVES;
DO PRADO, RENATA FALCHETE;
NEVES JARDINI, MARIA APARECIDA;
OLIVEIRA DE VASCONCELLOS, LUIS GUSTAVO;
CARVALHO, YASMIN RODARTE.
Porous Titanium Associated with CaP Coating: In Vivo and In Vitro Osteogenic Performance.
MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS,
Web of Science Citations: 1.