Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine

Autor(es)

Kohori, Natalia Akemi ^[1] ; Leme da Silva, Martin Kassio ^[1] ; Cesarino, Ivana ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Agr, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Solid State Electrochemistry; v. 22, n. 1, p. 141-148, JAN 2018.
• Citações Web of Science: 5

Resumo

Bioelectrodes were developed based on a simple deposition of graphene oxide (GO) or reduced graphed oxide (rGO) and laccase (Lac) on a glassy carbon (GC) electrode surface. The morphology and electrochemical behavior of the biosensors were characterized by scanning electron microscopy and cyclic voltammetry. These results demonstrated that only rGO was successfully applied for the immobilization of the laccase enzyme, improving the analytical signal for the determination of dopamine. The GC/rGO/Lac biosensor was applied to the detection of dopamine in synthetic urine and plasmatic serum samples, achieving a detection limit of 91.0 nmol L-1. (AU)

• Processo FAPESP: 15/02136-2 - Desenvolvimento de materiais avançados baseados em grafeno e nanopartículas metálicas para a análise de antibióticos em efluentes
• Beneficiário: Ivana Cesarino
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/09383-5 - Avaliação do compósito polipirrol-grafeno na imobilização de enzimas e sua aplicação como biossensor para neurotransmissores
• Beneficiário: Natália Akemi Kohori
• Linha de fomento: Bolsas no Brasil - Iniciação Científica