Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine

Author(s)

Kohori, Natalia Akemi ^[1] ; Leme da Silva, Martin Kassio ^[1] ; Cesarino, Ivana ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Agr, Botucatu, SP - Brazil

• Document type: Journal article
• Source: Journal of Solid State Electrochemistry; v. 22, n. 1, p. 141-148, JAN 2018.
• Web of Science Citations: 5

Abstract

Bioelectrodes were developed based on a simple deposition of graphene oxide (GO) or reduced graphed oxide (rGO) and laccase (Lac) on a glassy carbon (GC) electrode surface. The morphology and electrochemical behavior of the biosensors were characterized by scanning electron microscopy and cyclic voltammetry. These results demonstrated that only rGO was successfully applied for the immobilization of the laccase enzyme, improving the analytical signal for the determination of dopamine. The GC/rGO/Lac biosensor was applied to the detection of dopamine in synthetic urine and plasmatic serum samples, achieving a detection limit of 91.0 nmol L-1. (AU)

• FAPESP's process: 15/09383-5 - Evaluation of polypyrrole-graphene composite in the immobilization of enzymes and its application as a biosensor for neurotransmitters
• Grantee: Natália Akemi Kohori
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/02136-2 - Development of advanced materials based on graphene and metal nanoparticles for analysis of antibiotic in wastewater
• Grantee: Ivana Cesarino
• Support Opportunities: Regular Research Grants