Evaluation of polypyrrole-graphene composite in the immobilization of enzymes and its application as a biosensor for neurotransmitters

• Grant number: 15/09383-5
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2015
• Effective date (End): August 31, 2016
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal researcher: Ivana Cesarino
• Grantee: Natália Akemi Kohori
• Home Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

In this research project, the immobilization of laccase enzyme using polypyrrole-graphene composite will be evaluated employing the electrocodeposition method. The method has the benefit of depositing all components simultaneously, simple and fast. The biosensor proposed will be applied on neurotransmitters determination, in special dopamine. Neurotransmitters are extremely important for our organisms, acting on nervous system, being related to very recurrent diseases. The dopamine is deeply linked to illness like Parkinson’s disease, schizophrenia and depression. Thus, the graphene, one allotrope recently discovered from the graphite, will be used in the biosensor preparation by their high electrical conductivity properties and polypyrrole will be employed because it's a stable conductive polymer. Furthermore, synergism of polypyrrole-graphene eliminates the use of linker agents, improving the biosensor applicability and sensitivity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KOHORI, NATALIA AKEMI; LEME DA SILVA, MARTIN KASSIO; CESARINO, IVANA. Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine. Journal of Solid State Electrochemistry, v. 22, n. 1, p. 141-148, JAN 2018. Web of Science Citations: 5.