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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine

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Kohori, Natalia Akemi [1] ; Leme da Silva, Martin Kassio [1] ; Cesarino, Ivana [1]
Total Authors: 3
[1] Sao Paulo State Univ UNESP, Sch Agr, Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Solid State Electrochemistry; v. 22, n. 1, p. 141-148, JAN 2018.
Web of Science Citations: 5

Bioelectrodes were developed based on a simple deposition of graphene oxide (GO) or reduced graphed oxide (rGO) and laccase (Lac) on a glassy carbon (GC) electrode surface. The morphology and electrochemical behavior of the biosensors were characterized by scanning electron microscopy and cyclic voltammetry. These results demonstrated that only rGO was successfully applied for the immobilization of the laccase enzyme, improving the analytical signal for the determination of dopamine. The GC/rGO/Lac biosensor was applied to the detection of dopamine in synthetic urine and plasmatic serum samples, achieving a detection limit of 91.0 nmol L-1. (AU)

FAPESP's process: 15/02136-2 - Development of advanced materials based on graphene and metal nanoparticles for analysis of antibiotic in wastewater
Grantee:Ivana Cesarino
Support type: Regular Research Grants
FAPESP's process: 15/09383-5 - Evaluation of polypyrrole-graphene composite in the immobilization of enzymes and its application as a biosensor for neurotransmitters
Grantee:Natália Akemi Kohori
Support type: Scholarships in Brazil - Scientific Initiation