Development of advanced nanomaterials-based biosensors for viral detection.

• Grant number: 22/11894-1
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2023
• End date: October 31, 2025
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Ana Melva Champi Farfán
• Grantee: Ana Melva Champi Farfán
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated researchers: Helena Beatriz de Carvalho Ruthner Batista ; Ivana Barros de Campos ; Ricardo Moratelli Mendonça da Rocha

Abstract

Advanced nanomaterials are known to be smart materials, that is, they can be manipulated externally or functionalized with each other, modifying their physicochemical properties. A very interesting fact that also occurs is its easy functionalization with biological systems for biomedical applications, specifically in the biomonitoring of viral diseases through the development of biosensors from these materials. Among the advanced nanomaterials to be used in this project are those derived from graphene, TiO2 nanotubes, carbon dots and metallic nanoparticles.In particular, we will start with electrochemical biosensors based on graphene and derived materials such as graphene oxides (GO) and reduced graphene oxides (RGO). These nanomaterials, being biocompatible, have excellent reception with organic molecules such as DNA, enzymes and viral antibodies, due to the À-À bonds, hydrogen bonds, Van der Walls type bonds, electrostatics, among others, which allows us to measure the electrochemical current when interacts with viruses or viral RNA. Recently, we developed an RGO-based electrode with which a prototype of the biosensor proposed in this project was developed for applications in the detection of RABV virus RNA. Said electrode has a patent application number INPI BR 1020210139811, title "ELECTRODES BASED ON REDUCED GRAPHENE OXIDES FOR VIRAL DETECTION APPLICATIONS" - National Institute of Industrial Property. Brazil. Date: 07/15/2021. Due to recent experience and results Innovators of our research group in this area, we have managed to develop a portable platform based on Graphene derivatives for the detection of biological systems such as: RNA and viral DNA in partnership with Instituto Pasteur/SP and Fundação Osvaldo Cruz - FioCruz - RJ for tests with the biological samples with which we already have an important publication in Biosensors and Bioelectronics, 2023, on the proponent's coordination.Thus, in this project the proponent would like to explore and deepen the functionalization of TiO2 nanotubes, carbon dots and/or metallic and/or magnetic nanoparticles with Graphene derivatives to study the physical, chemical and biological effects on the electrodes of works developed with these nanomaterials, when exposed to biological systems such as viruses and/or bacteria, for which it would be of great value to be able to count on the resources of this Research Foundation. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHALLHUA, RONALDO; PRATI, RONALDO; CHAMPI, ANA. Feature engineering and machine learning for electrochemical detection of rabies virus in graphene-based biosensors. Microchemical Journal, v. 204, p. 8-pg., 2024-07-09. (22/11894-1)

DEMARCHI, ENRICO F.; GABRIEL, LAIS P.; CHAMPI, ANA; PEREIRA, ANA L. C.. Polycaprolactone Fibrous Membranes Coated with Reduced Graphene Oxide for Scalable 3D Printing of Electronics and Bioelectronics. ACS APPLIED NANO MATERIALS, v. 8, n. 14, p. 10-pg., 2025-04-02. (22/11894-1)

RONDAN, WILFREDO; DOS REIS, ROBERTA ALBINO; ACUNA, JOSE JAVIER SAEZ; SEABRA, AMEDEA BAROZZI; CHAMPI, ANA. Influence of multilayers Bernal and Rhombohedral graphene obtained by green chemistry on the acceleration in the germination process of tomato seeds. DIAMOND AND RELATED MATERIALS, v. 145, p. 9-pg., 2024-04-18. (22/11894-1, 22/00321-0)

RIVERA, MARIA; AKASHI, LARISSA; RODRIGUEZ, LUIS M.; PEREZ, PABLO D.; BARBOZA, CAMILA MOSCA; BATISTA, HELENA; CHAMPI, ANA. Potentiality graphene oxides-functionalized magnetic nanomaterials for RNA extraction from rabies and SARS-CoV-2 viruses. DIAMOND AND RELATED MATERIALS, v. 148, p. 10-pg., 2024-08-27. (22/11894-1)