A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms

Autor(es)

Chaves, Michel R. B. ^[1] ; Lima, Maria L. S. O. ^[1] ; Malafatti-Picca, Lusiane ^[2] ; de Angelis, Derlene A. ^[3] ; de Castro, Aline M. ^[4] ; Valoni, Erika ^[4] ; Marsaioli, Anita J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Quim, POB 6154, BR-13084971 Campinas, SP - Brazil

^[2] Univ Estadual Paulista Unesp, Ctr Estudos Ambientais, Av 24 A, 1515, BR-13506900 Rio Claro, SP - Brazil

^[3] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agrico, Div Recursos Microbianos, Rua Alexandre Cazellato 999, BR-13148218 Paulinia, SP - Brazil

^[4] Petrobras SA, Dept Biotecnol, Ctr R&D, Av Horacio Macedo 950, BR-21941915 Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 29, n. 6, p. 1278-1285, JUN 2018.
• Citações Web of Science: 1

Resumo

We propose a practical, low-cost and selective fluorescence-based protocol adapted to identify polyethylene terephthalate (PET) degrading microorganisms. The microbial hydrolysis of PET nanoparticles was monitored by 2-hydroxyterephthalate, a fluorophore produced in situ after radical hydroxylation of terephthalic acid (TPA), the final hydrolysis product, by the Fenton reaction. Seven fungi presenting promising PET hydrolytic potential using the proposed microscale screening assay were identified. The strains evaluated presented a substantial increase of up to 18-fold in PET nanoparticles conversion, such as obtained by the fungus Trichoderma sp. C70, after their cultivation in a PET-enriched medium. The formation of other hydrolysis products, along with TPA, was observed using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). (AU)

• Processo FAPESP: 12/00327-7 - Joint World Congress of ISPGR and Gait & Mental Function
• Beneficiário: Renato de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Reunião - Exterior
• Processo FAPESP: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock
• Beneficiário: Arlene Gonçalves Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia