Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks

Autor(es):Mostrar menos -

Martini, Matheus C. ^[1] ; Caserta, Leonardo C. ^[1] ; dos Santos, Marcia M. A. B. ^[2] ; Barnabe, Ana C. S. ^[1] ; Duraes-Carvalho, Ricardo ^[1] ; Padilla, Marina A. ^[1] ; Simao, Raphael M. ^[3] ; Rizotto, Lais S. ^[3] ; Simas, Paulo V. M. ^[1] ; Bastos, Juliana C. S. ^[1] ; Cardoso, Tereza C. ^[4] ; Felippe, Paulo A. N. ^[1] ; Ferreira, Helena L. ^{[3, 5]} ; Arns, Clarice W. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Lab Anim Virol, Campinas, SP - Brazil

^[2] Univ Fed Juiz de Fora, ICB, Juiz De Fora, MG - Brazil

^[3] Univ Sao Paulo, FMVZ, Fac Vet Med & Anim Sci, Postgrad Program Expt Epidemiol Zoonoses, Sao Paulo, SP - Brazil

^[4] Univ Estadual Paulista, Coll Vet Med, Lab Anim Virol & Cell Culture, DAPSA Dept, Aracatuba, SP - Brazil

^[5] Univ Sao Paulo, FZEA, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AVIAN PATHOLOGY; v. 47, n. 3, p. 286-293, 2018.
• Citações Web of Science: 0

Resumo

The detection of avian coronaviruses (AvCoV) in wild birds and the emergence of new AvCoV have increased in the past few years. In the present study, the pathogenicity of three AvCoV isolates was investigated in day-old chicks. One AvCoV isolated from a pigeon, which clustered with the Massachusetts vaccine serotype, and two AvCoV isolated from chickens, which grouped with a Brazilian genotype lineage, were used. Clinical signs, gross lesions, histopathological changes, ciliary activity, viral RNA detection, and serology were evaluated during 42 days post infection. All AvCoV isolates induced clinical signs, gross lesions in the trachea, moderate histopathological changes in the respiratory tract, and mild changes in other tissues. AvCoV isolated from the pigeon sample caused complete tracheal ciliostasis over a longer time span. Specific viral RNA was detected in all tissues, but the highest RNA loads were detected in the digestive tract (cloacal swabs and ileum). The highest antibody levels were also detected in the group infected with an isolate from the pigeon. These results confirm the pathogenicity of Brazilian variants, which can cause disease and induce gross lesions and histopathological changes in chickens. Our results suggest that non-Galliformes birds can also play a role in the ecology of AvCoV. (AU)

• Processo FAPESP: 11/50919-5 - Investigação de agentes virais na biota de aves silvestres no estado de São Paulo
• Beneficiário: Clarice Weis Arns
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 14/50696-4 - Dissecting essential roles of chicken interferon stimulated genes in the pathobiology of poultry viruses
• Beneficiário: Clarice Weis Arns
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/11510-5 - Desvendando as interações patógeno-hospedeiro e patógeno-patógeno utilizando abordagens de metagenômica viral e sequenciamento de última geração
• Beneficiário: Helena Lage Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular