Astaxanthin Restrains Nitrative-Oxidative Peroxidation in Mitochondrial-Mimetic Liposomes: A Pre-Apoptosis Model

Autor(es)

Mano, Camila M. ^{[1, 2, 3]} ; Guaratini, Thais ^{[2, 4]} ; Cardozo, Karina H. M. ^{[2, 5]} ; Colepicolo, Pio ^[2] ; Bechara, Etelvino J. H. ^{[6, 7]} ; Barros, Marcelo P. ^{[1, 8]}

Afiliação do(s) autor(es)

^[1] Univ Cruzeiro Sul, ICAFE, BR-01506000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo IQUSP, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil

^[3] Superintendencia Policia Tecn Cient, BR-05507060 Sao Paulo, SP - Brazil

^[4] Lychnoflora Pesquisa & Dev Prod Nat LTDA, BR-14030090 Ribeirao Preto, SP - Brazil

^[5] Grp Fleury, BR-04344070 Sao Paulo, SP - Brazil

^[6] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, UNIFESP, BR-09972270 Diadema, SP - Brazil

^[7] Univ Sao Paulo IQUSP, Inst Quim, Dept Quim Fundamental, BR-05508000 Sao Paulo, SP - Brazil

^[8] CSIC, IATA, Dept Ciencia Alimentos, Calle Catedrat Agustin Escardino 7, Paterna 46980 - Spain

• Tipo de documento: Artigo Científico
• Fonte: MARINE DRUGS; v. 16, n. 4 APR 2018.
• Citações Web of Science: 2

Resumo

Astaxanthin (ASTA) is a ketocarotenoid found in many marine organisms and that affords many benefits to human health. ASTA is particularly effective against radical-mediated lipid peroxidation, and recent findings hypothesize a ``mitochondrial-targeted{''} action of ASTA in cells. Therefore, we examined the protective effects of ASTA against lipid peroxidation in zwitterionic phosphatidylcholine liposomes (PCLs) and anionic phosphatidylcholine: phosphatidylglycerol liposomes (PCPGLs), at different pHs (6.2 to 8.0), which were challenged by oxidizing/nitrating conditions that mimic the regular and preapoptotic redox environment of active mitochondria. Pre-apoptotic conditions were created by oxidized/nitr(osyl) ated cytochrome c and resulted in the highest levels of lipoperoxidation in both PCL and PCPGLs (pH 7.4). ASTA was less protective at acidic conditions, especially in anionic PCPGLs. Our data demonstrated the ability of ASTA to hamper oxidative and nitrative events that lead to cytochrome c-peroxidase apoptosis and lipid peroxidation, although its efficiency changes with pH and lipid composition of membranes. (AU)

• Processo FAPESP: 17/06032-2 - Estratégias bioquímicas, fisiológicas e transcriptômicas para o estudo da relação entre carotenóides, estresse oxidativo e qualidade de frutas cítricas
• Beneficiário: Marcelo Paes de Barros
• Modalidade de apoio: Bolsas no Exterior - Pesquisa