Development and validation of an analytical method for multiresidue determination of pesticides in lettuce using QuEChERS-UHPLC-MS/MS

Autor(es)

Begnini Konatu, Fernanda Ribeiro ^[1] ; Sales Fontes Jardim, Isabel Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Quim, Caixa Postal 6154, BR-13084971 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SEPARATION SCIENCE; v. 41, n. 8, p. 1726-1733, APR 2018.
• Citações Web of Science: 13

Resumo

A new analytical method for multiresidue determination of 16 multiclass pesticides in lettuce was developed using ultra-high performance liquid chromatography with tandem mass spectrometry with a triple quadrupole mass analyzer and positive mode electrospray ionization, using a previously optimized quick, easy, cheap, effective, rugged, and safe method for sample preparation. Validation studies, according to document SANTE/11945/2015, demonstrated that the developed method is selective, accurate, and precise, providing recoveries of 70-120%, relative standard deviations <= 20% and quantification limits from 3 mu g/kg. The method was compared with one based on high-performance liquid chromatography with tandem mass spectrometry, in terms of chromatographic performance, detectability and matrix effect for five varieties of lettuce. The new method provided a reduction in the time for the chromatographic analysis of 50%, from 30 to 15 min, using a lower mobile phase flow rate (0.147 mL/min), which reduced the consumption of mobile phase by 25%, and injection of smaller amounts of sample (1.7 mu L). Lower limits of quantification were obtained for almost all pesticides studied for green-leaf lettuce. However, in relation to the matrix effect, four of the five types of lettuce studied presented higher matrix effects. (AU)

• Processo FAPESP: 11/23127-0 - Desenvolvimento e validação de métodos analíticos para determinação de multirresíduos de agrotóxicos em cultura de alface, por cromatografia líquida de ultra eficiência e cromatografia líquida acopladas à espectrometria de massas sequencial.
• Beneficiário: Fernanda Ribeiro Begnini
• Modalidade de apoio: Bolsas no Brasil - Doutorado