| Texto completo | |
| Autor(es): |
Murillo, Juliana Londono
[1]
;
Cabral, Aline Diniz
[1]
;
Uehara, Mabel
[1]
;
da Silva, Viviam Moura
[2]
;
dos Santos, Juliete Vitorino
[1]
;
Carvalho Muniz, Joao Renato
[3]
;
Estrozi, Leandro Farias
[4]
;
Fenel, Daphna
[4]
;
Garcia, Wanius
[2]
;
Speranca, Marcia Aparecida
[1]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Fed ABC, Ctr Cincias Nat & Humanas, Rua Arcturus 03, Jardim Antares, BR-09606070 Sao Paulo - Brazil
[2] Univ Fed ABC, Ctr Cincias Nat & Humanas, BR-09210170 Sao Paulo - Brazil
[3] Univ Sao Paulo, IFSC, Sao Carlos, SP - Brazil
[4] CEA, IBS, CNRS, F-38044 Grenoble - France
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Amino Acids; v. 50, n. 6, p. 711-721, JUN 2018. |
| Citações Web of Science: | 0 |
| Resumo | |
Oropouche virus (OROV) is the unique known human pathogen belonging to serogroup Simbu of Orthobunyavirus genus and Bunyaviridae family. OROV is transmitted by wild mosquitoes species to sloths, rodents, monkeys and birds in sylvatic environment, and by midges (Culicoides paraensis and Culex quinquefasciatus) to man causing explosive outbreaks in urban locations. OROV infection causes dengue fever-like symptoms and in few cases, can cause clinical symptoms of aseptic meningitis. OROV contains a tripartite negative RNA genome encapsidated by the viral nucleocapsid protein (NP), which is essential for viral genome encapsidation, transcription and replication. Here, we reported the first study on the structural properties of a recombinant NP from human pathogen Oropouche virus (OROV-rNP). OROV-rNP was successfully expressed in E. coli in soluble form and purified using affinity and size-exclusion chromatographies. Purified OROV-rNP was analyzed using a series of biophysical tools and molecular modeling. The results showed that OROV-rNP formed stable oligomers in solution coupled with endogenous E. coli nucleic acids (RNA) of different sizes. Finally, electron microscopy revealed a total of eleven OROV-rNP oligomer classes with tetramers (42%) and pentamers (43%) the two main populations and minor amounts of other bigger oligomeric states, such as hexamers, heptamers or octamers. The different RNA sizes and nucleotide composition may explain the diversity of oligomer classes observed. Besides, structural differences among bunyaviruses NP can be used to help in the development of tools for specific diagnosis and epidemiological studies of this group of viruses. (AU) | |
| Processo FAPESP: | 13/26096-4 - Expressão heteróloga da enzima quitinase de Leishmania (L.) infantum chagasi, Leishmania (V.) braziliensis e Leishmania amazonensis: diagnóstico sorológico e estudo molecular comparativo utilizando sistemas de expressão em células de insetos e bactéria |
| Beneficiário: | Aline Diniz Cabral |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 09/11347-6 - Arboviroses emergentes na Amazônia Ocidental: diagnóstico sorológico e molecular |
| Beneficiário: | Marcia Aparecida Speranca |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/03503-0 - Estudos da estabilidade, flexibilidade e atividade enzimática da beta-mananase da bactéria hipertermofílica Thermotoga petrophila |
| Beneficiário: | Viviam Moura da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 15/02897-3 - Análise estrutural e funcional do domínio fibronectina tipo III (FnIII) de uma beta-glicosidase da família GH3: interação com substratos poliméricos e termoestabilidade |
| Beneficiário: | Wanius José Garcia da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Regular |