| Full text | |
| Author(s): |
Murillo, Juliana Londono
[1]
;
Cabral, Aline Diniz
[1]
;
Uehara, Mabel
[1]
;
da Silva, Viviam Moura
[2]
;
dos Santos, Juliete Vitorino
[1]
;
Carvalho Muniz, Joao Renato
[3]
;
Estrozi, Leandro Farias
[4]
;
Fenel, Daphna
[4]
;
Garcia, Wanius
[2]
;
Speranca, Marcia Aparecida
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Fed ABC, Ctr Cincias Nat & Humanas, Rua Arcturus 03, Jardim Antares, BR-09606070 Sao Paulo - Brazil
[2] Univ Fed ABC, Ctr Cincias Nat & Humanas, BR-09210170 Sao Paulo - Brazil
[3] Univ Sao Paulo, IFSC, Sao Carlos, SP - Brazil
[4] CEA, IBS, CNRS, F-38044 Grenoble - France
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Amino Acids; v. 50, n. 6, p. 711-721, JUN 2018. |
| Web of Science Citations: | 0 |
| Abstract | |
Oropouche virus (OROV) is the unique known human pathogen belonging to serogroup Simbu of Orthobunyavirus genus and Bunyaviridae family. OROV is transmitted by wild mosquitoes species to sloths, rodents, monkeys and birds in sylvatic environment, and by midges (Culicoides paraensis and Culex quinquefasciatus) to man causing explosive outbreaks in urban locations. OROV infection causes dengue fever-like symptoms and in few cases, can cause clinical symptoms of aseptic meningitis. OROV contains a tripartite negative RNA genome encapsidated by the viral nucleocapsid protein (NP), which is essential for viral genome encapsidation, transcription and replication. Here, we reported the first study on the structural properties of a recombinant NP from human pathogen Oropouche virus (OROV-rNP). OROV-rNP was successfully expressed in E. coli in soluble form and purified using affinity and size-exclusion chromatographies. Purified OROV-rNP was analyzed using a series of biophysical tools and molecular modeling. The results showed that OROV-rNP formed stable oligomers in solution coupled with endogenous E. coli nucleic acids (RNA) of different sizes. Finally, electron microscopy revealed a total of eleven OROV-rNP oligomer classes with tetramers (42%) and pentamers (43%) the two main populations and minor amounts of other bigger oligomeric states, such as hexamers, heptamers or octamers. The different RNA sizes and nucleotide composition may explain the diversity of oligomer classes observed. Besides, structural differences among bunyaviruses NP can be used to help in the development of tools for specific diagnosis and epidemiological studies of this group of viruses. (AU) | |
| FAPESP's process: | 13/26096-4 - Heterologous expression of the chitinase enzyme of Leishmania (L.) infantum chagasi, Leishmania (V.) braziliensis and Leishmania (V.) amazonensis: serological diagnosis and comparative molecular study using expression systems of insects cell and bacteria |
| Grantee: | Aline Diniz Cabral |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 09/11347-6 - Emergent arboviruses in ocidental Amazon: molecular and sorologic diagnosis |
| Grantee: | Marcia Aparecida Speranca |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/03503-0 - Studies of stability, flexibility and enzymatic activity of the beta-mannanase from hyperthermophilic bacterium Thermotoga petrophila |
| Grantee: | Viviam Moura da Silva |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 15/02897-3 - Structural and functional analysis of the fibronectin type III domain (FnIII) of one beta-glucosidase belonging to the family GH3: interaction with large polymeric substrates and thermostability |
| Grantee: | Wanius José Garcia da Silva |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |