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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Characterization and engineering of a plastic-degrading aromatic polyesterase

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Autor(es):
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Austin, Harry P. [1] ; Allen, Mark D. [1] ; Donohoe, Bryon S. [2] ; Rorrer, Nicholas A. [3] ; Kearns, Fiona L. [4] ; Silveira, Rodrigo L. [5, 2] ; Pollard, Benjamin C. [4] ; Dominick, Graham [3] ; Duman, Ramona [6] ; El Omari, Kamel [6] ; Mykhaylyk, Vitaliy [6] ; Wagner, Armin [6] ; Michener, William E. [3] ; Amore, Antonella [2] ; Skaf, Munir S. [5] ; Crowley, Michael F. [2] ; Thorne, Alan W. [1] ; Johnson, Christopher W. [3] ; Woodcock, H. Lee [4] ; McGeehan, John E. [1] ; Beckham, Gregg T. [3]
Número total de Autores: 21
Afiliação do(s) autor(es):
[1] Univ Portsmouth, Inst Biol & Biomed Sci, Sch Biol Sci, Mol Biophys Labs, Portsmouth PO1 2DY, Hants - England
[2] Natl Renewable Energy Lab, Biosci Ctr, Golden, CO 80401 - USA
[3] Natl Renewable Energy Lab, Natl Bioenergy Ctr, Golden, CO 80401 - USA
[4] Univ S Florida, Dept Chem, Tampa, FL 33620 - USA
[5] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil
[6] Harwell Sci & Innovat Campus, Diamond Light Source, Didcot OX11 0DE, Oxon - England
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; v. 115, n. 19, p. E4350-E4357, MAY 8 2018.
Citações Web of Science: 49
Resumo

Poly(ethylene terephthalate) (PET) is one of the most abundantly produced synthetic polymers and is accumulating in the environment at a staggering rate as discarded packaging and textiles. The properties that make PET so useful also endow it with an alarming resistance to biodegradation, likely lasting centuries in the environment. Our collective reliance on PET and other plastics means that this buildup will continue unless solutions are found. Recently, a newly discovered bacterium, Ideonella sakaiensis 201-F6, was shown to exhibit the rare ability to grow on PET as a major carbon and energy source. Central to its PET biodegradation capability is a secreted PETase (PET-digesting enzyme). Here, we present a 0.92 angstrom resolution X-ray crystal structure of PETase, which reveals features common to both cutinases and lipases. PETase retains the ancestral alpha/beta-hydrolase fold but exhibits a more open active-site cleft than homologous cutinases. By narrowing the binding cleft via mutation of two active-site residues to conserved amino acids in cutinases, we surprisingly observe improved PET degradation, suggesting that PETase is not fully optimized for crystalline PET degradation, despite presumably evolving in a PET-rich environment. Additionally, we show that PETase degrades another semiaromatic polyester, polyethylene-2,5-furandicarboxylate (PEF), which is an emerging, bioderived PET replacement with improved barrier properties. In contrast, PETase does not degrade aliphatic polyesters, suggesting that it is generally an aromatic polyesterase. These findings suggest that additional protein engineering to increase PETase performance is realistic and highlight the need for further developments of structure/activity relationships for biodegradation of synthetic polyesters. (AU)

Processo FAPESP: 16/22956-7 - Simulações de QM/MM híbridas de feruloil esterases: mecanismo de clivagem de complexos lignina-carboidrato em paredes celulares de plantas
Beneficiário:Rodrigo Leandro Silveira
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
Processo FAPESP: 14/10448-1 - Aspectos moleculares da arquitetura de paredes celulares de plantas
Beneficiário:Rodrigo Leandro Silveira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/08293-7 - CECC - Centro de Engenharia e Ciências Computacionais
Beneficiário:Munir Salomao Skaf
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs