| Texto completo | |
| Autor(es): |
Dias Panariello, Beatriz Helena
[1]
;
Klein, Marlise I.
[1]
;
Pavarina, Ana Claudia
[1]
;
Duarte, Simone
[2]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ Unesp, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara - Brazil
[2] Indiana Univ, Sch Dent, Dept Cariol Operat Dent & Dent Publ Hlth, Indianapolis, IN 46202 - USA
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF ORAL MICROBIOLOGY; v. 9, OCT 17 2017. |
| Citações Web of Science: | 6 |
| Resumo | |
Background: Infections caused by Candida spp. have been associated with formation of a biofilm, i.e. a complex microstructure of cells adhering to a surface and embedded within an extracellular matrix (ECM). Methods: The ECMs of a wild-type (WT, SN425) and two Candida albicans mutant strains, Delta/Delta tec1 (CJN2330) and Delta/Delta efg1 (CJN2302), were evaluated. Colony-forming units (cfu), total biomass (mg), water-soluble polysaccharides (WSPs), alkali-soluble polysaccharides (ASPs), proteins (insoluble part of biofilms and matrix proteins), and extracellular DNA (eDNA) were quantified. Variable-pressure scanning electron microscopy and confocal scanning laser microscopy were performed. The biovolume (mu m(3)/mu m(2)) and maximum thickness (mu m) of the biofilms were quantified using COMSTAT2. Results: ASP content was highest in WT (mean +/- SD: 74.5 +/- 22.0 mu g), followed by Delta/Delta tec1 (44.0 +/- 24.1 mu g) and Delta/Delta efg1 (14.7 +/- 5.0 mu g). The protein correlated with ASPs (r = 0.666) and with matrix proteins (r = 0.670) in the WT strain. The population in Delta/Delta efg1 correlated with the protein (r = 0.734) and its biofilms exhibited the lowest biomass and biovolume, and maximum thickness. In Delta/Delta tec1, ASP correlated with eDNA (r = 0.678). Conclusion: ASP production may be linked to C. albicans cell filamentous morphology. (AU) | |
| Processo FAPESP: | 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida |
| Beneficiário: | Vanderlei Salvador Bagnato |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 16/00256-3 - A matriz extracelular de biofilmes de Candida albicans e Candida glabrata |
| Beneficiário: | Beatriz Helena Dias Panariello |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 14/18804-1 - Matriz extracelular de biofilmes de Candida albicans e Candida glabrata susceptíveis e resistentes ao fluconazol: Caracterização, ação de enzimas hidrolíticas e eficácia da associação das enzimas à Terapia Fotodinâmica. |
| Beneficiário: | Beatriz Helena Dias Panariello |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |