Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology

Full text
Author(s):
Dias Panariello, Beatriz Helena [1] ; Klein, Marlise I. [1] ; Pavarina, Ana Claudia [1] ; Duarte, Simone [2]
Total Authors: 4
Affiliation:
[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara - Brazil
[2] Indiana Univ, Sch Dent, Dept Cariol Operat Dent & Dent Publ Hlth, Indianapolis, IN 46202 - USA
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF ORAL MICROBIOLOGY; v. 9, OCT 17 2017.
Web of Science Citations: 6
Abstract

Background: Infections caused by Candida spp. have been associated with formation of a biofilm, i.e. a complex microstructure of cells adhering to a surface and embedded within an extracellular matrix (ECM). Methods: The ECMs of a wild-type (WT, SN425) and two Candida albicans mutant strains, Delta/Delta tec1 (CJN2330) and Delta/Delta efg1 (CJN2302), were evaluated. Colony-forming units (cfu), total biomass (mg), water-soluble polysaccharides (WSPs), alkali-soluble polysaccharides (ASPs), proteins (insoluble part of biofilms and matrix proteins), and extracellular DNA (eDNA) were quantified. Variable-pressure scanning electron microscopy and confocal scanning laser microscopy were performed. The biovolume (mu m(3)/mu m(2)) and maximum thickness (mu m) of the biofilms were quantified using COMSTAT2. Results: ASP content was highest in WT (mean +/- SD: 74.5 +/- 22.0 mu g), followed by Delta/Delta tec1 (44.0 +/- 24.1 mu g) and Delta/Delta efg1 (14.7 +/- 5.0 mu g). The protein correlated with ASPs (r = 0.666) and with matrix proteins (r = 0.670) in the WT strain. The population in Delta/Delta efg1 correlated with the protein (r = 0.734) and its biofilms exhibited the lowest biomass and biovolume, and maximum thickness. In Delta/Delta tec1, ASP correlated with eDNA (r = 0.678). Conclusion: ASP production may be linked to C. albicans cell filamentous morphology. (AU)

FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/00256-3 - The extracellular matrix of Candida albicans and Candida glabrata biofilms
Grantee:Beatriz Helena Dias Panariello
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 14/18804-1 - Extracellular matrix of susceptible and fluconazole-resistant Candida albicans and Candida glabrata biofilms: characterization, action of hydrolytic enzymes and efficacy of the combination of enzymes to Photodynamic Therapy.
Grantee:Beatriz Helena Dias Panariello
Support Opportunities: Scholarships in Brazil - Doctorate