Oocyte oxidative DNA damage may be involved in minimal/mild endometriosis-related infertility

Autor(es)

Da Broi, Michele G. ^[1] ; Jordao-, Jr., Alceu A. ^[2] ; Ferriani, Rui A. ^{[1, 3]} ; Navarro, Paula A. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Obstet & Gynecol, Human Reprod Div, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Nutr & Metab Lab, Sao Paulo - Brazil

^[3] CNPq, Natl Inst Hormones & Womans Hlth, Porto Alegre, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular Reproduction and Development; v. 85, n. 2, p. 128-136, FEB 2018.
• Citações Web of Science: 8

Resumo

Early endometriosis is associated with infertility, and oxidative stress may play a role in the pathogenesis of disease-related infertility. This prospective case-control study aimed to compare the presence of oxidative stress markers in the follicular microenvironment and systemic circulation of infertile women with minimal/mild endometriosis (EI/II) versus individuals undergoing controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI). Seventy-one blood samples (27 from infertile women with EI/II and 44 controls with tubal and/or male infertility factor) and 51 follicular fluid samples (19 EI/II and 32 controls) were obtained on the day of oocyte retrieval. Total hydroperoxides (FOX1), reduced glutathione, vitamin E, Superoxide dismutase, total antioxidant capacity, malondialdehyde, advanced oxidation protein products, and 8-hydroxy-2-deoxyguanosine (8OHdG) concentrations were measured in both fluids. Women with EI/II showed higher FOX1 (8.48 +/- 1.72 vs. 7.69 +/- 1.71mol/g protein) and lower total antioxidant capacity (0.38 +/- 0.18 vs. 0.46 +/- 0.15mEq Trolox/L) concentrations in serum, and higher 8OHdG concentrations (24.21 +/- 8.56 vs. 17.22 +/- 5.6ng/ml) in follicular fluid compared with controls. These data implicate both systemic and follicular oxidative stress may in infertile women with EI/II undergoing controlled ovarian stimulation for ICSI. Furthermore, the elevated 8OHdG concentrations in follicular fluid of women with EI/II may be related to compromised oocyte quality. (AU)

• Processo FAPESP: 14/15866-6 - Annual Meeting of the American Society for Reproductive Medicine - ASRM2014
• Beneficiário: Paula Andrea de Albuquerque Salles Navarro
• Modalidade de apoio: Auxílio à Pesquisa - Reunião - Exterior
• Processo FAPESP: 08/58197-6 - Balanço oxidante e antioxidante de mulheres inférteis com endometriose comparadas a controle (fatores masculino e tubário de infertilidade) submetidas a estimulação ovariana e influência nos resultados
• Beneficiário: Paula Andrea de Albuquerque Salles Navarro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/56373-1 - Avaliação da peroxidação lipídica, dos níveis de vitamina E e da capacidade antioxidante total no soro de mulheres inférteis com endometriose e infertilidade por fatores tubário e masculino e associação com a resposta à estimulação e os resultados da injeção intracitoplasmática de espermatozoides
• Beneficiário: Rafaela Lopes Cardoso
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica