Increased chemical acetylation of peptides and proteins in rats after daily ingestion of diacetyl analyzed by Nano-LC-MS/MS

Autor(es)

Lima Jedlicka, Leticia Dias ^{[1, 2]} ; Guterres, Sheila Barreto ^{[1, 3]} ; Balbino, Aleksandro Martins ^[1] ; Neto, Giuseppe Bruno ^[1] ; Landgraf, Richardt Gama ^[1] ; Fernandes, Liliam ^[1] ; Carrilho, Emanuel ^[4] ; Henriques Bechara, Etelvino Jose ^{[1, 5]} ; Assuncao, Nilson A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Diadema, SP - Brazil

^[2] Univ Fed Sul & Sudeste Para, Inst Studies Hlth & Biol, Collect Hlth, Maraba, PA - Brazil

^[3] Fundacao Univ Fed Rondonia, Dept Chem, Porto Velho, RO - Brazil

^[4] Univ Sao Paulo, Sao Carlos Inst Chem, Sao Carlos, SP - Brazil

^[5] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PeerJ; v. 6, APR 25 2018.
• Citações Web of Science: 2

Resumo

Background. Acetylation alters several protein properties including molecular weight, stability, enzymatic activity, protein protein interactions, and other biological functions. Our previous findings demonstrating that diacetyl/peroxynitrite can acetylate L-lysine, L-histidine, and albumin in vitro led us to investigate whether diacetyl-treated rats suffer protein acetylation as well. Methods. Wistar rats were administered diacetyl daily for four weeks, after which they were sacrificed, and their lung proteins were extracted to be analysed by Nano-LC-MS/MS (Q-TOF). A C18 reversed-phase colurnn and gradient elution with formic acid/acetonitrile solutions from 2 to 50% over 150 min were used to separate the proteins. Protein detection was performed using a microTOE-Q II (QTOF) equipped with captive source and an electrospray-ionization source. The data frommass spectrometry were processed using a Compass 1.7 and analyzed using Protein Scape, software that uses Mascot algorithms to perform protein searches. Results. A set of 3,162 acetylated peptides derived from 351 acetylated proteins in the diacetyl-treated group was identified. Among them, 23 targeted proteins were significantly more acetylated in the diacetyl-treated group than in the PBS control. Protein acetylation of the group treated with 540 mg/kg/day of diacetyl was corroborated by Western blotting analysis. Conclusions. These data support our hypothesis that diacetyl exposure in animals may lead to the generation of acetyl radicals, compounds that attach to proteins, affecting their functions and triggering adverse health problems. (AU)

• Processo FAPESP: 13/07763-0 - Estudos da Acetilação de Proteínas Via Radicalar Empregando Ferramentas Proteomicas
• Beneficiário: Leticia Dias Lima Jedlicka
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/02514-9 - Uso de técnicas proteômicas para o estudo da acetilação de proteínas via radicalar desencadeada pelos produtos da reação diacetil/peroxinitrito
• Beneficiário: Nilson Antonio de Assunção
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/01404-0 - Estudos in vivo e in vitro da participação da leptina em diferentes modelos de inflamação pulmonar: mediadores inflamatórios e vias de sinalização envolvidas
• Beneficiário: Richardt Gama Landgraf
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores