Chronic treatment with fluoride affects the jejunum: insights from proteomics and enteric innervation analysis

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Dionizio, Aline Salgado ^[1] ; Souza Melo, Carina Guimaraes ^[1] ; Sabino-Arias, Isabela Tomazini ^[1] ; Silva Ventura, Talita Mendes ^[1] ; Leite, Aline Lima ^[1] ; Garcia Souza, Sara Raquel ^[2] ; Santos, Erika Xavier ^[2] ; Heubel, Alessandro Domingues ^[1] ; Souza, Juliana Gadelha ^[1] ; Colombo Martins Perles, Juliana Vanessa ^[2] ; Zanoni, Jacqueline Nelisis ^[2] ; Rabelo Buzalaf, Marilia Afonso ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Sci Biol, Bauru - Brazil

^[2] Univ Estadual Maringa, Dept Morphophysiol Sci, Maringa, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 8, FEB 16 2018.
• Citações Web of Science: 3

Resumo

Gastrointestinal symptoms are the first signs of fluoride (F) toxicity. In the present study, the jejunum of rats chronically exposed to F was evaluated by proteomics, as well as by morphological analysis. Wistar rats received water containing 0, 10 or 50 mgF/L during 30 days. HuC/D, neuronal Nitric Oxide (nNOS), Vasoactive Intestinal Peptide (VIP), Calcitonin Gene Related Peptide (CGRP), and Substance P (SP) were detected in the myenteric plexus of the jejunum by immunofluorescence. The density of nNOS-IR neurons was significantly decreased (compared to both control and 10 mgF/L groups), while the VIP-IR varicosities were significantly increased (compared to control) in the group treated with the highest F concentration. Significant morphological changes were seen observed in the density of HUC/D-IR neurons and in the area of SP-IR varicosities for F-treated groups compared to control. Changes in the abundance of various proteins correlated with relevant biological processes, such as protein synthesis, glucose homeostasis and energy metabolism were revealed by proteomics. (AU)

• Processo FAPESP: 16/09100-6 - Análise proteômica do jejuno e íleo em ratos expostos a dose aguda ou crônica de fluoreto.
• Beneficiário: Aline Dionizio Valle
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/10233-7 - Avaliação da inervação entérica e análise proteômica do intestino delgado de ratos expostos a dose aguda ou crônica de fluoreto
• Beneficiário: Carina Guimarães de Souza Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/16840-5 - Toxicologia do fluoreto: estudos proteômicos em intestino e fígado
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular