Chronic treatment with fluoride affects the jejunum: insights from proteomics and enteric innervation analysis

Author(s):Show less -

Dionizio, Aline Salgado ^[1] ; Souza Melo, Carina Guimaraes ^[1] ; Sabino-Arias, Isabela Tomazini ^[1] ; Silva Ventura, Talita Mendes ^[1] ; Leite, Aline Lima ^[1] ; Garcia Souza, Sara Raquel ^[2] ; Santos, Erika Xavier ^[2] ; Heubel, Alessandro Domingues ^[1] ; Souza, Juliana Gadelha ^[1] ; Colombo Martins Perles, Juliana Vanessa ^[2] ; Zanoni, Jacqueline Nelisis ^[2] ; Rabelo Buzalaf, Marilia Afonso ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Sci Biol, Bauru - Brazil

^[2] Univ Estadual Maringa, Dept Morphophysiol Sci, Maringa, Parana - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 8, FEB 16 2018.
• Web of Science Citations: 3

Abstract

Gastrointestinal symptoms are the first signs of fluoride (F) toxicity. In the present study, the jejunum of rats chronically exposed to F was evaluated by proteomics, as well as by morphological analysis. Wistar rats received water containing 0, 10 or 50 mgF/L during 30 days. HuC/D, neuronal Nitric Oxide (nNOS), Vasoactive Intestinal Peptide (VIP), Calcitonin Gene Related Peptide (CGRP), and Substance P (SP) were detected in the myenteric plexus of the jejunum by immunofluorescence. The density of nNOS-IR neurons was significantly decreased (compared to both control and 10 mgF/L groups), while the VIP-IR varicosities were significantly increased (compared to control) in the group treated with the highest F concentration. Significant morphological changes were seen observed in the density of HUC/D-IR neurons and in the area of SP-IR varicosities for F-treated groups compared to control. Changes in the abundance of various proteins correlated with relevant biological processes, such as protein synthesis, glucose homeostasis and energy metabolism were revealed by proteomics. (AU)

• FAPESP's process: 16/09100-6 - Proteomic analysis of the jejunum and ileum in rats exposed to chronic or acute doses of fluoride.
• Grantee: Aline Dionizio Valle
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/10233-7 - Evaluation of enteric innervation and proteomic analysis of small intestin of rats exposed to chronic or acute fluoride dose
• Grantee: Carina Guimarães de Souza Melo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/16840-5 - Fluoride toxicology: proteomic studies in gut and liver
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants