Multilamellar-to-Unilamellar Transition Induced by Diphenylalanine in Lipid Vesicles

Autor(es)

Gerbelli, Barbara Bianca ^[1] ; da Silva, Emerson Rodrigo ^[2] ; Soares, Bruna Miranda ^[3] ; Alves, Wendel Andrade ^[3] ; de Oliveira, Elisabeth Andreoli ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Fis, BR-05314970 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biofis, BR-04023062 Sao Paulo - Brazil

^[3] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Langmuir; v. 34, n. 5, p. 2171-2179, FEB 6 2018.
• Citações Web of Science: 3

Resumo

In the present work, we investigate the effect of two short phenylalanine-based peptides on lipid membranes. A simplified model membrane composed of lecithin vesicles was used to incorporate different amounts of the two amino acid sequences, the dimmer L,L-diphenylallanine (FF) and the trimmer cysteine-diphenylallanine (CFF). Spectroscopic and scattering techniques were applied to probe in detail the structural behavior of lipid membranes in the presence of the peptides. The experimental results demonstrate that both peptides are located mainly at the interface of the membrane interacting with phosphate groups modifying membrane thickness and flexibility. The multilamellar structure of the vesicles is preserved with inclusion of small amounts of FF, accompanied by changes in membrane thickness and elasticity. Finally, a multi-to unilamellar transition is observed as a result of peptide self-association into a crystalline structure onto the membrane interface. (AU)

• Processo FAPESP: 16/24409-3 - Peptídeos Penetrantes de Células para Transporte de DNA plasmídial e microRNA: da Estrutura Nanoscópica a Entrega de Genes.
• Beneficiário: Emerson Rodrigo da Silva
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/24018-1 - Conjugados polímero-peptídeo para formulação de hidrogéis e seu uso em detecção
• Beneficiário: Wendel Andrade Alves
• Linha de fomento: Auxílio à Pesquisa - Regular