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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Immobilization Effects on the Catalytic Properties of Two Fusarium Verticillioides Lipases: Stability, Hydrolysis, Transesterification and Enantioselectivity Improvement

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Autor(es):
Antonio Facchini, Fernanda Dell [1] ; Pereira, Marita Gimenez [2] ; Vici, Ana Claudia [2] ; Filice, Marco [3] ; Costa Pessela, Benevides [4] ; Manuel Guisan, Jose [5] ; Fernandez-Lorente, Gloria [4] ; Teixeira de Moraes Polizeli, Maria de Lourdes [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Med, Fac Med Ribeirao Preto, BR-14040900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Sao Paulo - Brazil
[3] Spanish Natl Ctr Cardiovasc Res, CNIC, Nanobiotechnol Mol Imaging & Metabol Grp, Madrid 28029 - Spain
[4] CSIC, CIAL, Inst Ciencias Alimentac, Dept Biotecnol & Microbiol Alimentos, Calle Nicolas Cabrera 9, CampusUAM, Canto Blanco 28049 - Spain
[5] CSIC, Inst Catalisis & Petroleoquim, Dept Biocatalisis, Campus UAM, Canto Blanco 28049 - Spain
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: CATALYSTS; v. 8, n. 2 FEB 2018.
Citações Web of Science: 4
Resumo

Fusarium verticillioides lipases were purified in a ``cascade{''} method using octadecyl Sepabeads and octyl Sepharose resins, which led to the isolation of two proteins with lipolytic activities. Lip 1 was purified after octyl Sepharose adsorption presenting 30.3 kDa and, Lip 2 presented 68.0 kDa after octadecyl adsorption. These immobilization processes resulted in an increase of 3-fold in activity of each immobilized enzyme. These enzymes presented optima of pH of 5.0 and 6.0, respectively and temperature at 40 degrees C. They were thermostable at 40 degrees C and both remained more than 50% of its activity at the pH range of 5.0 to 7.0, with 180 min of incubation. The sardine oil hydrolysis showed higher EPA/DHA ratio. Concerning the ethanolysis reaction, Lip 2 showed higher conversion (5.5%) and both lipases showed activity in the release of the S enantiomers from 2-O-butyryl-2-phenylacetic acid (mandelic butyrate acid) and HPBE hydrolysis. Lip 2 also demonstrated capacity of transesterification. These applications made these enzymes attractive for industrial application. (AU)

Processo FAPESP: 13/50892-5 - Desenvolvimento de estratégias de imobilização e o uso de enzimas como biocatalizadores para aplicações industriais: produção de biodiesel e ingredientes funcionais
Beneficiário:Maria de Lourdes Teixeira de Moraes Polizeli
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/52322-3 - Bioprospecção de fungos filamentosos produtores de holoenzimas com aplicação em biorefinaria
Beneficiário:Maria de Lourdes Teixeira de Moraes Polizeli
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular