| Full text | |
| Author(s): |
Antonio Facchini, Fernanda Dell
[1]
;
Pereira, Marita Gimenez
[2]
;
Vici, Ana Claudia
[2]
;
Filice, Marco
[3]
;
Costa Pessela, Benevides
[4]
;
Manuel Guisan, Jose
[5]
;
Fernandez-Lorente, Gloria
[4]
;
Teixeira de Moraes Polizeli, Maria de Lourdes
[2]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Dept Med, Fac Med Ribeirao Preto, BR-14040900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Sao Paulo - Brazil
[3] Spanish Natl Ctr Cardiovasc Res, CNIC, Nanobiotechnol Mol Imaging & Metabol Grp, Madrid 28029 - Spain
[4] CSIC, CIAL, Inst Ciencias Alimentac, Dept Biotecnol & Microbiol Alimentos, Calle Nicolas Cabrera 9, CampusUAM, Canto Blanco 28049 - Spain
[5] CSIC, Inst Catalisis & Petroleoquim, Dept Biocatalisis, Campus UAM, Canto Blanco 28049 - Spain
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | CATALYSTS; v. 8, n. 2 FEB 2018. |
| Web of Science Citations: | 4 |
| Abstract | |
Fusarium verticillioides lipases were purified in a ``cascade{''} method using octadecyl Sepabeads and octyl Sepharose resins, which led to the isolation of two proteins with lipolytic activities. Lip 1 was purified after octyl Sepharose adsorption presenting 30.3 kDa and, Lip 2 presented 68.0 kDa after octadecyl adsorption. These immobilization processes resulted in an increase of 3-fold in activity of each immobilized enzyme. These enzymes presented optima of pH of 5.0 and 6.0, respectively and temperature at 40 degrees C. They were thermostable at 40 degrees C and both remained more than 50% of its activity at the pH range of 5.0 to 7.0, with 180 min of incubation. The sardine oil hydrolysis showed higher EPA/DHA ratio. Concerning the ethanolysis reaction, Lip 2 showed higher conversion (5.5%) and both lipases showed activity in the release of the S enantiomers from 2-O-butyryl-2-phenylacetic acid (mandelic butyrate acid) and HPBE hydrolysis. Lip 2 also demonstrated capacity of transesterification. These applications made these enzymes attractive for industrial application. (AU) | |
| FAPESP's process: | 13/50892-5 - Development of strategies of immobilization and use of enzymes as biocatalysis for industrial applications: production of biodiesel and functional ingredients |
| Grantee: | Maria de Lourdes Teixeira de Moraes Polizeli |
| Support Opportunities: | Regular Research Grants |